[艾宁体外诱导人胃腺癌细胞SGC-7901凋亡及细胞周期的研究]
[Study on apoptosis of human gastric adenocarcinoma cell SGC-7901 induced by aining in vitro and the cell cycle].
作者信息
Su M, Lü X, Li H, Wan B, Liu J
机构信息
National Engineering Research Center for Mordernization of TCM, Zhuhai 519020.
出版信息
Zhong Yao Cai. 2001 Dec;24(12):876-9.
OBJECTIVE
To study the inducing apoptosis effect of Aining on human gastric adenocarcinoma cell in vitro.
METHODS
Agarose gel electrophoresis (AGE) and flow cytometry (FMC) techniques were adopted to study the inducing apoptosis effect of Ainging on the human gastric adenocarcinoma cell.
RESULTS
The human gastric adenocarcinoma cell's DNA was digested into fragments which gave rise to a characteristic "DNA Ladder" on agarose gel electrophoresis, while the percentage of apoptotic cells was 43.5%. But the blank group had neither DNA ladder, nor apoptotic peak. Cell cycle analysis showed that the proliferation index was 33.7% after the human gastric adenocarcinoma cells treated with Aining, and there was no cell phase specialty.
CONCLUSION
Aining can induce human gastric adenocarcinoma cell apoptosis in vitro.
目的
研究艾宁对人胃腺癌细胞的体外诱导凋亡作用。
方法
采用琼脂糖凝胶电泳（AGE）和流式细胞术（FMC）技术研究艾宁对人胃腺癌细胞的诱导凋亡作用。
结果
人胃腺癌细胞的DNA被消化成片段，在琼脂糖凝胶电泳上产生特征性的“DNA梯带”，而凋亡细胞的百分比为43.5%。但空白组既无DNA梯带，也无凋亡峰。细胞周期分析显示，艾宁处理后人胃腺癌细胞的增殖指数为33.7%，且无细胞周期特异性。
结论
艾宁可在体外诱导人胃腺癌细胞凋亡。
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