哮喘患者血清和诱导痰中TH2型CC趋化因子胸腺和活化调节趋化因子（TARC）水平升高。
Increased levels of a TH2-type CC chemokine thymus and activation-regulated chemokine (TARC) in serum and induced sputum of asthmatics.
作者信息
Sekiya T, Yamada H, Yamaguchi M, Yamamoto K, Ishii A, Yoshie O, Sano Y, Morita A, Matsushima K, Hirai K
机构信息
Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine, Tokyo, Japan.
出版信息
Allergy. 2002 Feb;57(2):173-7. doi: 10.1034/j.1398-9995.2002.5720256.x.
BACKGROUND
Cytokines liberated by TH2 cells play crucial roles in the pathogenesis of bronchial asthma. Recent studies have demonstrated that CC chemokine receptor (CCR)4 is preferentially expressed by TH2 cells. These facts suggest possible involvement of two CCR4-specific ligands i.e., thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC), in the pathogenesis of bronchial asthma via recruitment of TH2 cells to inflammatory sites. We investigated the levels of TARC and MDC in the serum and induced sputum of asthmatics.
METHODS
The levels of TARC in the serum (46 asthmatics and 26 healthy subjects) and induced sputum (30 asthmatics and 6 healthy subjects) were measured using a highly sensitive ELISA system. The levels of eotaxin and MDC were also measured by ELISA.
RESULTS
TARC, but not MDC, was significantly increased in asthmatic sera (P<0.001). Although MDC was undetectable in the sputum of most cases by our assay system, sputum TARC was significantly increased (P=0.027).
CONCLUSIONS
The elevated TARC levels in asthmatics might be involved in the pathophysiology of asthma.
背景
TH2细胞释放的细胞因子在支气管哮喘的发病机制中起关键作用。最近的研究表明，CC趋化因子受体（CCR）4在TH2细胞中优先表达。这些事实提示两种CCR4特异性配体，即胸腺和活化调节趋化因子（TARC）和巨噬细胞衍生趋化因子（MDC），可能通过将TH2细胞募集到炎症部位而参与支气管哮喘的发病机制。我们研究了哮喘患者血清和诱导痰中TARC和MDC的水平。
方法
使用高灵敏度ELISA系统检测血清（46例哮喘患者和26例健康受试者）和诱导痰（30例哮喘患者和6例健康受试者）中TARC的水平。嗜酸性粒细胞趋化因子和MDC的水平也通过ELISA检测。
结果
哮喘患者血清中TARC显著升高（P<0.001），而MDC未升高。虽然在我们的检测系统中大多数病例的痰中未检测到MDC，但痰中TARC显著升高（P=0.027）。
结论
哮喘患者TARC水平升高可能参与哮喘的病理生理过程。
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