Yao Wei, Qian Guisheng, Yang Xiaojing
Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Chin Med J (Engl). 2002 Jan;115(1):107-9.
To evaluate the roles of Na+/H+ exchanger-1 (NHE-1) in the proliferation and apoptosis of pulmonary artery smooth muscle cells in rats.
Twenty Wistar rats were randomized into control group and 3-week hypoxic group. Intracellular pH (pHi) of the smooth muscle was determined with fluorescence measurement of the pH-sensitive dye BCECF-AM, and the expression of NHE-1 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Primary culture of pulmonary artery smooth muscle cells in vitro was performed. In situ cell death detection kit (TUNEL) was used for studying the effect of specific NHE-1 inhibitor-dimethyl amiloride (DMA) on the apoptosis of muscle cells which had intracellular acidification.
pHi value and NHE-1 mRNA expression of pulmonary artery smooth muscle cells were significantly higher in the hypoxic group than in the control group (P < 0.01, P < 0.001). DMA elevated the apoptotic ratio remarkably. The effect was enhanced when DMA concentration increased and the time prolonged.
With the function of adjusting pHi, NHE-1 may play an important role in the proliferation and apoptosis of pulmonary artery smooth muscle cells.
评估钠氢交换体1（NHE-1）在大鼠肺动脉平滑肌细胞增殖和凋亡中的作用。
将20只Wistar大鼠随机分为对照组和3周低氧组。用pH敏感染料BCECF-AM的荧光测量法测定平滑肌细胞内pH（pHi），用逆转录聚合酶链反应（RT-PCR）检测NHE-1 mRNA的表达。进行肺动脉平滑肌细胞的体外原代培养。使用原位细胞死亡检测试剂盒（TUNEL）研究特异性NHE-1抑制剂二甲基氨氯吡脒（DMA）对发生细胞内酸化的肌肉细胞凋亡的影响。
低氧组肺动脉平滑肌细胞的pHi值和NHE-1 mRNA表达显著高于对照组（P<0.01，P<0.001）。DMA显著提高凋亡率。随着DMA浓度增加和时间延长，该作用增强。
NHE-1具有调节pHi的功能，可能在肺动脉平滑肌细胞的增殖和凋亡中起重要作用。
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