Preservation of splenic immune functions by female sex hormones after trauma-hemorrhage.

OBJECTIVES

Immune functions are markedly depressed in males but not in proestrus females after trauma-hemorrhage. Nonetheless, it is unclear what role sex steroids play in the maintenance of immune function in females after trauma-hemorrhage.

DESIGN

Prospective, controlled animal study.

SETTING

University research laboratory.

SUBJECTS

Eight-week-old female CBA/J mice.

INTERVENTIONS

Mice underwent sham-ovariectomy or ovariectomy. Two weeks thereafter, ovariectomized and proestrus sham-ovariectomized mice were subjected to laparotomy (i.e., soft tissue trauma) and hemorrhagic shock (35 +/- 5 mm Hg for 90 mins, resuscitated) or sham operation. Splenocyte proliferation and interleukin-2, interleukin-3, and interferon-gamma release were determined at 2 hrs after trauma-hemorrhage.

MEASUREMENTS AND MAIN RESULTS

These immune functional capacities were maintained in proestrus sham-ovariectomized mice after trauma-hemorrhage, whereas they were suppressed in ovariectomized mice subjected to trauma-hemorrhage. 17beta-Estradiol in vitro had no effect on splenocyte functions in proestrus sham-ovariectomized females; however, addition of 17beta-estradiol to splenocytes from ovariectomized females subjected to trauma-hemorrhage normalized immune functional capacities.

CONCLUSIONS

These findings suggest that elevated circulating 17beta-estradiol in proestrus females plays a direct role in the maintenance of immunocompetence after trauma-hemorrhage.