Vastmans Karen, Rozenski Jef, Van Aerschot Arthur, Herdewijn Piet
Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Louvain, Belgium.
Biochim Biophys Acta. 2002 May 20;1597(1):115-22. doi: 10.1016/s0167-4838(02)00267-4.
Hexitol nucleic acids (HNA) as well as their 1,5-anhydrohexitol triphosphate building blocks were evaluated for their ability to be recognized by several DNA metabolizing enzymes. It was found that RNA polymerases can recognize the triphosphate of the adenine analogue. However, only the incorporation of a maximum of three consecutive building block analogues was possible under the applied experimental conditions. Terminal transferase was more successful succeeding in the elongation of a DNA primer with a maximum of 15 1,5-anhydrohexitol purine nucleotides. Furthermore, it was observed that the 1,5-anhydroaltritol triphosphate analogue of adenosine was a poor substrate for terminal transferase and that HNA could not act as a primer for this enzyme. Likewise, HNA did not function as a template for restriction enzymes, ligases or methylases.
对己糖醇核酸（HNA）及其1,5-脱水己糖醇三磷酸构建块被几种DNA代谢酶识别的能力进行了评估。发现RNA聚合酶能够识别腺嘌呤类似物的三磷酸。然而，在所应用的实验条件下，最多只能掺入三个连续的构建块类似物。末端转移酶更为成功，能够用最多15个1,5-脱水己糖醇嘌呤核苷酸成功延长DNA引物。此外，观察到腺苷的1,5-脱水阿卓糖醇三磷酸类似物是末端转移酶的不良底物，并且HNA不能作为该酶的引物。同样，HNA也不能作为限制性内切酶、连接酶或甲基化酶的模板。
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