基于全基因组鸟枪法测序得到的小鼠16号染色体与人类基因组的比较。

A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome.

作者信息

Mural Richard J, Adams Mark D, Myers Eugene W, Smith Hamilton O, Miklos George L Gabor, Wides Ron, Halpern Aaron, Li Peter W, Sutton Granger G, Nadeau Joe, Salzberg Steven L, Holt Robert A, Kodira Chinnappa D, Lu Fu, Chen Lin, Deng Zuoming, Evangelista Carlos C, Gan Weiniu, Heiman Thomas J, Li Jiayin, Li Zhenya, Merkulov Gennady V, Milshina Natalia V, Naik Ashwinikumar K, Qi Rong, Shue Bixiong Chris, Wang Aihui, Wang Jian, Wang Xin, Yan Xianghe, Ye Jane, Yooseph Shibu, Zhao Qi, Zheng Liansheng, Zhu Shiaoping C, Biddick Kendra, Bolanos Randall, Delcher Arthur L, Dew Ian M, Fasulo Daniel, Flanigan Michael J, Huson Daniel H, Kravitz Saul A, Miller Jason R, Mobarry Clark M, Reinert Knut, Remington Karin A, Zhang Qing, Zheng Xiangqun H, Nusskern Deborah R, Lai Zhongwu, Lei Yiding, Zhong Wenyan, Yao Alison, Guan Ping, Ji Rui-Ru, Gu Zhiping, Wang Zhen-Yuan, Zhong Fei, Xiao Chunlin, Chiang Chia-Chien, Yandell Mark, Wortman Jennifer R, Amanatides Peter G, Hladun Suzanne L, Pratts Eric C, Johnson Jeffery E, Dodson Kristina L, Woodford Kerry J, Evans Cheryl A, Gropman Barry, Rusch Douglas B, Venter Eli, Wang Mei, Smith Thomas J, Houck Jarrett T, Tompkins Donald E, Haynes Charles, Jacob Debbie, Chin Soo H, Allen David R, Dahlke Carl E, Sanders Robert, Li Kelvin, Liu Xiangjun, Levitsky Alexander A, Majoros William H, Chen Quan, Xia Ashley C, Lopez John R, Donnelly Michael T, Newman Matthew H, Glodek Anna, Kraft Cheryl L, Nodell Marc, Ali Feroze, An Hui-Jin, Baldwin-Pitts Danita, Beeson Karen Y, Cai Shuang, Carnes Mark, Carver Amy, Caulk Parris M, Center Angela, Chen Yen-Hui, Cheng Ming-Lai, Coyne My D, Crowder Michelle, Danaher Steven, Davenport Lionel B, Desilets Raymond, Dietz Susanne M, Doup Lisa, Dullaghan Patrick, Ferriera Steven, Fosler Carl R, Gire Harold C, Gluecksmann Andres, Gocayne Jeannine D, Gray Jonathan, Hart Brit, Haynes Jason, Hoover Jeffery, Howland Tim, Ibegwam Chinyere, Jalali Mena, Johns David, Kline Leslie, Ma Daniel S, MacCawley Steven, Magoon Anand, Mann Felecia, May David, McIntosh Tina C, Mehta Somil, Moy Linda, Moy Mee C, Murphy Brian J, Murphy Sean D, Nelson Keith A, Nuri Zubeda, Parker Kimberly A, Prudhomme Alexandre C, Puri Vinita N, Qureshi Hina, Raley John C, Reardon Matthew S, Regier Megan A, Rogers Yu-Hui C, Romblad Deanna L, Schutz Jakob, Scott John L, Scott Richard, Sitter Cynthia D, Smallwood Michella, Sprague Arlan C, Stewart Erin, Strong Renee V, Suh Ellen, Sylvester Karena, Thomas Reginald, Tint Ni Ni, Tsonis Christopher, Wang Gary, Wang George, Williams Monica S, Williams Sherita M, Windsor Sandra M, Wolfe Keriellen, Wu Mitchell M, Zaveri Jayshree, Chaturvedi Kabir, Gabrielian Andrei E, Ke Zhaoxi, Sun Jingtao, Subramanian Gangadharan, Venter J Craig, Pfannkoch Cynthia M, Barnstead Mary, Stephenson Lisa D

机构信息

Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.

出版信息

Science. 2002 May 31;296(5573):1661-71. doi: 10.1126/science.1069193.

DOI:10.1126/science.1069193

PMID:12040188

Abstract

The high degree of similarity between the mouse and human genomes is demonstrated through analysis of the sequence of mouse chromosome 16 (Mmu 16), which was obtained as part of a whole-genome shotgun assembly of the mouse genome. The mouse genome is about 10% smaller than the human genome, owing to a lower repetitive DNA content. Comparison of the structure and protein-coding potential of Mmu 16 with that of the homologous segments of the human genome identifies regions of conserved synteny with human chromosomes (Hsa) 3, 8, 12, 16, 21, and 22. Gene content and order are highly conserved between Mmu 16 and the syntenic blocks of the human genome. Of the 731 predicted genes on Mmu 16, 509 align with orthologs on the corresponding portions of the human genome, 44 are likely paralogous to these genes, and 164 genes have homologs elsewhere in the human genome; there are 14 genes for which we could find no human counterpart.

摘要

通过对小鼠16号染色体（Mmu 16）序列的分析，证明了小鼠和人类基因组之间的高度相似性。该序列是小鼠基因组全基因组鸟枪法组装的一部分。由于重复DNA含量较低，小鼠基因组比人类基因组小约10%。将Mmu 16的结构和蛋白质编码潜力与人类基因组的同源片段进行比较，确定了与人类3号、8号、12号、16号、21号和22号染色体（Hsa）存在保守同线性的区域。Mmu 16与人类基因组的同线性区域之间的基因含量和顺序高度保守。在Mmu 16上预测的731个基因中，509个与人类基因组相应部分的直系同源基因对齐，44个可能与这些基因是旁系同源的，164个基因在人类基因组的其他地方有同源物；有14个基因我们在人类基因组中找不到对应物。