两株显示疣状毛癣菌线粒体DNA类型的贝氏节皮菌分离株。

Two arthroderma benhamiae isolates showing mitochondrial DNA type of Trichophyton verrucosum.

作者信息

Kawasaki Masako, Inoue Tadanori, Ohsawa Tokuya, Ishioka Sakae, Mochizuki Takashi, Ishizaki Hiroshi

机构信息

Department of Dermatology, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan.

出版信息

Nihon Ishinkin Gakkai Zasshi. 2002;43(2):103-6. doi: 10.3314/jjmm.43.103.

DOI:10.3314/jjmm.43.103

PMID:12040369

Abstract

Two Trichophyton mentagrophytes isolates from skin lesions on a girl and her rabbit, which were identified as Arthroderma benhamiae by mating tests, were studied by mtDNA (mitochondrial DNA) analysis and sequencing of ITS (internal transcribed spacer) regions of nuclear ribosomal RNA genes. The mtDNA-RFLP (restriction fragment length polymorphism) patterns showed the two isolates to be T. verrucosum rather than A. benhamiae. The ITS sequence showed the isolates to be closer to A. benhamiae Americano-European race than to T. verrucosum and closer to T. verrucosum than to their mating partner A. benhamiae African race. These results suggest that there are three genetic types of A. benhamiae - an Americano-European type, an African type and a T. verrucosum type - and that T. verrucosum is an anamorph of A. benhamiae.

摘要

从一名女孩及其兔子的皮肤损伤处分离出的两株须癣毛癣菌，经交配试验鉴定为贝氏节皮菌，通过线粒体DNA（mtDNA）分析和核糖体RNA基因内部转录间隔区（ITS）测序对其进行了研究。mtDNA限制性片段长度多态性（RFLP）图谱显示这两株分离株为疣状毛癣菌而非贝氏节皮菌。ITS序列显示，这些分离株与贝氏节皮菌欧美种族的亲缘关系比与疣状毛癣菌更近，且与疣状毛癣菌的亲缘关系比与其交配型伙伴贝氏节皮菌非洲种族更近。这些结果表明，贝氏节皮菌存在三种遗传类型——欧美型、非洲型和疣状毛癣菌型——并且疣状毛癣菌是贝氏节皮菌的无性型。