Awuah R T, Ellis W O
Department of Crop Science, Kwame Nkrumah University of Science of Technology, Kumasi, Ghana.
Mycopathologia. 2002;154(1):29-36. doi: 10.1023/a:1015259518448.
Powders from the leaves of Ocimum gratissimum and cloves of Syzygium aromaticum were used as protectants at 3% (w/w) in combination with various packaging methods to store 3.5 kg groundnut kernel samples (9.3% moisture) artificially inoculated with Aspergillus parasiticus. Phostoxin-protected and unprotected samples were the controls. Packaging was accomplished with (i) Jute bags; JB (ii) Interlaced polypropylene bags; IPPB (iii) Polyethylene bags; PB (iv) PB inserted into IPPB and (v) PB inserted into JB. Selected treatments were repeated concurrently with naturally infected kernels (6.6% moisture). With 9.3% moisture kernels, there was a highly significant protectant, packaging method, and protectant X packaging method effect on protection of kernels from fungal infection at 2, 4, and 6 months. Packaging with JB and IPPB with or without plant powders gave 100% protection against fungi but insect infestation was prevented only when the Syzygium powder was used. When PB was used either singly or in combination with JB and IPPB, 100% protection from fungi was achieved up to 2 months with the Ocimum and up to 4 months with the Syzygium powder. The phostoxin treatment also gave 100% protection with JB and IPPB packaging but was ineffective with PB packaging. Kernels packaged with PB without the powders were extensively mouldy. Kernels with natural mycoflora (6.6% moisture) were free from fungi at 6 months regardless of the protectant and packaging used. In further tests, the Syzygium powder, at 3% and in combination with JB-packaging, effectively suppressed cross infection of healthy kernels (12% moisture) by fungi from diseased kernels when both kernel types occurred in the same lot. At 18.5% kernel moisture and with identical packaging, the Syzygium powder at 3%, was not as effective.
将九层塔叶片粉末和丁香粉末按3%(w/w)的比例用作保护剂,并结合各种包装方法,来储存人工接种寄生曲霉的3.5千克花生仁样品(含水量9.3%)。磷化铝处理过的和未处理的样品作为对照。包装方式有:(i)黄麻袋;JB(ii)交织聚丙烯袋;IPPB(iii)塑料袋;PB(iv)PB插入IPPB中;(v)PB插入JB中。选定的处理方法与自然感染的果仁(含水量6.6%)同时重复进行。对于含水量9.3%的果仁,在2个月、4个月和6个月时,保护剂、包装方法以及保护剂X包装方法对果仁免受真菌感染的保护作用具有极显著差异。用JB和IPPB包装,无论有无植物粉末,都能100%防止真菌感染,但只有使用丁香粉末时才能防止昆虫侵害。当单独使用PB或与JB和IPPB组合使用时,使用九层塔粉末在2个月内、丁香粉末在4个月内可实现100%防止真菌感染。磷化铝处理在使用JB和IPPB包装时也能提供100%的保护,但对PB包装无效。未加粉末用PB包装的果仁发霉严重。对于带有自然菌群(含水量6.6%)的果仁而言,无论使用何种保护剂和包装方式,6个月时均未感染真菌。在进一步的试验中,3%的丁香粉末与JB包装结合使用时,当健康果仁(含水量12%)和患病果仁在同一批次中时,能有效抑制患病果仁上的真菌对健康果仁的交叉感染。在果仁含水量为18.5%且包装相同的情况下,3%的丁香粉末效果不佳。