Kampfer Peter, Neef Alexander, Salkinoja-Salonen Mirja S, Buss Hans-Jürgen
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität, Giessen, Germany.
Int J Syst Evol Microbiol. 2002 May;52(Pt 3):835-9. doi: 10.1099/00207713-52-3-835.
Chelatobacter heintzii, which was described as a nitrilotriacetate-utilizing organism, was re-investigated in order to clarify its taxonomic position. On the basis of 16S rDNA sequence comparisons, it is obvious that this species clusters phylogenetically with species of the genus Aminobacter. The results of investigations of the fatty acid patterns, polar lipid profiles, polyamine patterns and quinone system supported this placement. The substrate-utilization profiles and fatty acid patterns of four strains (belonging to two different genomovars) revealed homogeneous results and showed high levels of similarity to Aminobacter aminovorans. DNA-DNA similarity studies confirmed that both genomovars of Chelatobacter heintzii belong to Aminobacter aminovorans. It could be shown that all species of this group are highly interrelated. On the basis of these data and previously published results, it is obvious that Chelatobacter heintzii is a later subjective synonym of Aminobacter aminovorans.
海因茨螯合杆菌最初被描述为一种利用次氮基三乙酸的微生物,为了明确其分类地位,对其进行了重新研究。基于16S rDNA序列比较,很明显该物种在系统发育上与氨基杆菌属的物种聚类在一起。脂肪酸模式、极性脂质谱、多胺模式和醌系统的研究结果支持了这一分类。四株菌株(属于两个不同的基因组变种)的底物利用谱和脂肪酸模式显示出一致的结果,并且与氨基营养氨基杆菌具有高度相似性。DNA-DNA相似性研究证实,海因茨螯合杆菌的两个基因组变种均属于氨基营养氨基杆菌。可以看出,该类群的所有物种都高度相关。基于这些数据和先前发表的结果,很明显海因茨螯合杆菌是氨基营养氨基杆菌的一个较晚出现的主观同义词。