异担子菌中双链RNA的传播
Transmission of double-stranded RNA in Heterobasidion annosum.
作者信息
Ihrmark Katarina, Johannesson Hanna, Stenström Elna, Stenlid Jan
机构信息
Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, SE-750 07 Uppsala, Sweden.
出版信息
Fungal Genet Biol. 2002 Jul;36(2):147-54. doi: 10.1016/S1087-1845(02)00011-7.
Transmission of dsRNA viruses between homo- and heterokaryotic mycelia paired on agar plates and into conidia has been studied in Heterobasidion annosum. Horizontal transmission of dsRNA occurred between both homo- and heterokaryotic isolates, as well as between isolates belonging to different intersterility groups. The proportions of vertical transmission into conidia were 3% and 55%, respectively, for the two isolates included in the study. RT-PCR of dsRNA and PCR-RFLP of mitochondrial markers were used to confirm transmission of dsRNA between the cytoplasms of different mycelia. The identity of nuclei and nuclear migration during experiments were verified using PCR-RFLP of several nuclear markers.
在松白腐菌中，研究了双链RNA病毒在琼脂平板上配对的同核体和异核体菌丝体之间的传播以及向分生孢子中的传播。双链RNA在同核体和异核体分离株之间以及属于不同不育组的分离株之间均发生水平传播。该研究中包含的两个分离株向分生孢子的垂直传播比例分别为3%和55%。双链RNA的逆转录聚合酶链反应（RT-PCR）和线粒体标记的聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）被用于确认双链RNA在不同菌丝体细胞质之间的传播。实验过程中细胞核的同一性和核迁移通过几种核标记的PCR-RFLP进行了验证。
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