利用LightCycler系统从BacT/ALERT血培养瓶中快速鉴定金黄色葡萄球菌和mecA基因。
Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.
作者信息
Shrestha Nabin K, Tuohy Marion J, Hall Gerri S, Isada Carlos M, Procop Gary W
机构信息
Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
出版信息
J Clin Microbiol. 2002 Jul;40(7):2659-61. doi: 10.1128/JCM.40.7.2659-2661.2002.
Abstract
One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.
摘要
对100瓶培养出呈簇状革兰氏阳性球菌的BacT/ALERT血培养瓶进行培养，并通过LightCycler PCR对sa442和mecA基因进行研究。PCR检测金黄色葡萄球菌及金黄色葡萄球菌中的耐甲氧西林情况时，敏感性和特异性均为100%，但检测凝固酶阴性葡萄球菌中的耐甲氧西林情况时准确性较低。
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本文引用的文献
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