体外受精-胚胎移植过程中人类黄素化颗粒细胞凋亡的预后意义
Prognostic implication of apoptosis in human luteinized granulosa cells during IVF-ET.
作者信息
Suh Chang Suk, Jee Byung Chul, Choi Young Min, Kim Jung Gu, Lee Jin Yong, Moon Shin Yong, Kim Seok Hyun
机构信息
Department of Obstetrics and Gynecology, College of Medicine, Institute of Reproductive Medicine and Populations, Medical Research Center, Seoul National University, South Korea.
出版信息
J Assist Reprod Genet. 2002 May;19(5):209-14. doi: 10.1023/a:1015319617598.
PURPOSE
To investigate the prognostic significance of apoptosis in granulosa cells recovered from patients participating in IVF-ET.
METHODS
Seventy-three women underwent ovarian hyperstimulation for IVF and embryo transfer. After follicle aspiration, recovered granulosa cells were stained by BCL2 monoclonal antibody with FITC and propidium iodide (PI). Fluorescence was detected by flowcytometry, then the apoptotic index (Al) and BCL2 positivity were assessed.
RESULTS
The pregnant group showed a significantly lower apoptotic index in granulosa cells compared with the nonpregnant group (p < 0.0001). Patient's age, basal serum FSH concentration, serum E2 concentrations at hCG day, number of retrieved oocytes, fertilization rates. number of embryos transferred, and BCL2 positivity were not different between the two groups. Linear regression analysis of AI to serum basal FSH showed a positive correlation. Al in granulosa cells of 6.14% or below could predict a successful pregnancy with a sensitivity of 87.5% and a specificity of 73.7%.
CONCLUSION
Our results indicate that apoptotic analysis within granulosa cells can be used as a prognostic indicator for IVF success.
目的
研究从接受体外受精-胚胎移植（IVF-ET）的患者中回收的颗粒细胞凋亡的预后意义。
方法
73名女性接受卵巢过度刺激以进行IVF和胚胎移植。卵泡抽吸后，回收的颗粒细胞用异硫氰酸荧光素（FITC）和碘化丙啶（PI）标记的BCL2单克隆抗体染色。通过流式细胞术检测荧光，然后评估凋亡指数（Al）和BCL2阳性率。
结果
与未怀孕组相比，怀孕组颗粒细胞的凋亡指数显著降低（p < 0.0001）。两组患者的年龄、基础血清促卵泡激素（FSH）浓度、人绒毛膜促性腺激素（hCG）日的血清雌二醇（E2）浓度、回收的卵母细胞数量、受精率、移植的胚胎数量和BCL2阳性率无差异。Al与血清基础FSH的线性回归分析显示呈正相关。颗粒细胞中Al低于6.14%可预测成功妊娠，敏感性为87.5%，特异性为73.7%。
结论
我们的结果表明，颗粒细胞内的凋亡分析可作为IVF成功的预后指标。
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