爱泼斯坦-巴尔病毒潜伏膜蛋白-1（LMP-1）在口腔鳞状细胞癌中的表达
Epstein-Barr virus latent membrane protein-1 (LMP-1) expression in oral squamous cell carcinoma.
作者信息
Gonzalez-Moles M A, Gutierrez J, Rodriguez M J, Ruiz-Avila I, Rodriguez-Archilla A
机构信息
Department of Oral Medicine, School of Dentistry, University of Granada, Spain.
出版信息
Laryngoscope. 2002 Mar;112(3):482-7. doi: 10.1097/00005537-200203000-00014.
OBJECTIVES
Epstein-Barr virus (EBV) is frequently associated with malignant cell transformation through the action of the oncoprotein latent membrane protein-1 (LMP-1). The present study aimed to determine the presence of EBV in oral squamous cell carcinomas (OSCCs) and the expression of LMP-1 in neoplastic cells of EBV-positive OSCCs.
STUDY DESIGN/METHODS: In a retrospective study of 78 OSCCs, we investigated the presence of the DNA of EBV by polymerase chain reaction, the expression of the oncoprotein LMP-1 by immunohistochemistry, and the presence of EBV-encoded RNA (EBER) by in situ hybridization.
RESULTS
EBV DNA was detected in 19.2% of the cases. Expression of LMP-1 in neoplastic cells was found in 85.7% of the EBV-positive OSCCs. EBV presence was significantly more frequent (P <.05) in OSCCs localized on the lateral tongue. EBV-positive OSCCs more frequently presented (P <.05) greater nuclear atypia.
CONCLUSION
EBV can appear in latent form in OSCC and express its main oncoprotein, LMP-1.
目的
爱泼斯坦-巴尔病毒（EBV）常通过癌蛋白潜伏膜蛋白1（LMP-1）的作用与恶性细胞转化相关。本研究旨在确定口腔鳞状细胞癌（OSCC）中EBV的存在情况以及EBV阳性OSCC肿瘤细胞中LMP-1的表达情况。
研究设计/方法：在一项对78例OSCC的回顾性研究中，我们通过聚合酶链反应研究EBV DNA的存在情况，通过免疫组织化学研究癌蛋白LMP-1的表达情况，并通过原位杂交研究EBV编码RNA（EBER）的存在情况。
结果
19.2%的病例检测到EBV DNA。在85.7%的EBV阳性OSCC中发现肿瘤细胞中有LMP-1表达。位于舌侧的OSCC中EBV的存在明显更频繁（P<.05）。EBV阳性OSCC更常出现（P<.05）更大的核异型性。
结论
EBV可在OSCC中以潜伏形式出现并表达其主要癌蛋白LMP-1。
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