大鼠急性损伤肺组织中AQP1和AQP5表达降低。
Decreased expression of AQP1 and AQP5 in acute injured lungs in rats.
作者信息
Jiao Guangyu, Li Erran, Yu Runjiang
机构信息
Institute of Respiratory Disease, The First Clinical Hospital, China Medical University, Shenyang 110001, China.
出版信息
Chin Med J (Engl). 2002 Jul;115(7):963-7.
OBJECTIVE
To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs.
METHODS
The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity-purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS).
RESULTS
Immunolabelling showed AQP1 was stained primarily in the microvascular endotheli a of normal lungs, while AQP5 was expressed in type I pneumocytes. Immunohisto chemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged.
CONCLUSION
The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.
目的
确定水通道蛋白1（AQP1）和水通道蛋白5（AQP5）是否在大鼠肺泡毛细血管膜中表达。此外，研究急性肺损伤时AQP1和AQP5的变化。
方法
采用抗人AQP1和AQP５的亲和纯化抗体，通过免疫组织化学和免疫电子显微镜研究AQP1和AQP5在肺泡毛细血管膜中的分布。为了研究肺泡毛细血管膜AQP1和AQP5调节改变的可能性，我们建立了经肺泡滴注脂多糖（LPS）的大鼠模型。
结果
免疫标记显示，AQP1主要在正常肺组织的微血管内皮细胞中染色，而AQP5在I型肺细胞中表达。免疫组织化学分析显示，LPS滴注后4小时至48小时，损伤肺组织中AQP1和AQP5的表达显著降低。LPS滴注和给予类固醇后24小时，AQP1蛋白部分恢复，而AQP5不变。
结论
损伤肺组织中AQP1和AQP5表达降低，提示它们可能在异常液体转运中起作用。
Zotero 插件