Wolle Kathlen, Leodolter Andreas, Malfertheiner Peter, König Wolfgang
Institute of Medical Microbiology and *Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke-University Magdeburg, Germany.
J Med Microbiol. 2002 Aug;51(8):705-709. doi: 10.1099/0022-1317-51-8-705.
The issue of antibiotic resistance in Helicobacter pylori is of particular concern and has become an important factor leading to eradication failure. This paper reports the prevalence of primary resistance to clarithromycin, amoxicillin, metronidazole and tetracycline among H. pylori isolates in the north-eastern part of Germany. A total of 1644 clinical H. pylori isolates was investigated over a period of 6 years from 1995 to 2000. The MICs were determined by the Etest. The overall rate of primary resistance was 26.2% for metronidazole and 2.2% for clarithromycin. No significant changes in the resistance rates during the period of investigation were observed. No isolate was resistant to amoxicillin or tetracycline. PCR-RFLP analysis for the detection of point mutations associated with clarithromycin resistance was performed with 36 H. pylori isolates. The A --> G transition mutation at position 2143 was detected in 19 H. pylori isolates (52.8%), whereas the mutation at position 2142 was found in 13 isolates (36.1%).
幽门螺杆菌的抗生素耐药性问题备受关注，已成为导致根除治疗失败的重要因素。本文报道了德国东北部幽门螺杆菌分离株对克拉霉素、阿莫西林、甲硝唑和四环素的原发性耐药率。在1995年至2000年的6年时间里，共对1644株临床幽门螺杆菌分离株进行了研究。采用Etest法测定最低抑菌浓度（MIC）。甲硝唑的原发性耐药总率为26.2%，克拉霉素为2.2%。在研究期间未观察到耐药率有显著变化。没有分离株对阿莫西林或四环素耐药。对36株幽门螺杆菌分离株进行了用于检测与克拉霉素耐药相关点突变的PCR-RFLP分析。在19株幽门螺杆菌分离株（52.8%）中检测到2143位的A→G转换突变，而在13株分离株（36.1%）中发现了2142位的突变。
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