排列在毛细管中的珠子上的DNA探针，即“珠阵列”，表现出高杂交性能。
DNA probes on beads arrayed in a capillary, 'Bead-array', exhibited high hybridization performance.
作者信息
Kohara Yoshinobu, Noda Hideyuki, Okano Kazunori, Kambara Hideki
机构信息
Central Research Laboratory, Hitachi Ltd, 1-280 Higashi-Koigakubo, Kokubunji, Tokyo 185-8601, Japan.
出版信息
Nucleic Acids Res. 2002 Aug 15;30(16):e87. doi: 10.1093/nar/gnf086.
A DNA analysis platform called 'Bead-array' is presented and its features when used in hybridization detection are shown. In 'Bead-array', beads of 100- micro m diameter are lined in a determined order in a capillary. Each bead is conjugated with DNA probes, and can be identified by its order in the capillary. This probe array is easily produced by just arraying beads conjugated with probes into the capillary in a fixed order. The hybridization is also easily completed by introducing samples (1-300 micro l) into the capillary with reciprocal flow. For hybridization detection, as little as 1 amol of fluorescent-labeled oligo DNA was detected. The hybridization reaction was completed in 1 min irrespective of the amount of target DNA. When the number of target molecules was smaller than that of probe molecules on the bead, 10 fmol, almost all targets were captured on the bead. 'Bead-array' enables reliable and reproducible measurement of the target quantity. This rapid and sensitive platform seems very promising for various genetic testing tasks.
介绍了一种名为“微珠阵列”的DNA分析平台，并展示了其用于杂交检测时的特点。在“微珠阵列”中，直径为100微米的微珠在毛细管中按确定顺序排列。每个微珠都与DNA探针结合，并可通过其在毛细管中的顺序进行识别。这种探针阵列通过将与探针结合的微珠以固定顺序排列到毛细管中即可轻松制备。通过以往复流动的方式将样品（1 - 300微升）引入毛细管，杂交也很容易完成。对于杂交检测，检测到的荧光标记寡聚DNA低至1阿托摩尔。无论靶DNA的量如何，杂交反应在1分钟内即可完成。当靶分子的数量小于微珠上探针分子的数量（10飞摩尔）时，几乎所有靶标都被捕获在微珠上。“微珠阵列”能够可靠且可重复地测量靶标量。这个快速且灵敏的平台对于各种基因检测任务似乎非常有前景。
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