VanWijk Marja J, Nieuwland Rienk, Boer Kees, van der Post Joris A M, VanBavel Ed, Sturk Augueste
Departments of Obstetrics and Gynecology, Academic Medical Center, Amsterdam, The Netherlands.
Am J Obstet Gynecol. 2002 Aug;187(2):450-6. doi: 10.1067/mob.2002.124279.
The purpose of this study was to investigate the cellular origin and numbers of circulating microparticles in normal pregnancy and preeclampsia.
Plasma samples from 10 women with preeclampsia, from 10 normal pregnant women, and from 10 nonpregnant women matched for age and gestation, were analyzed by flow cytometry.
The total number of circulating microparticles was unaltered in pregnancy and preeclampsia. The largest portion of microparticles was derived from platelets in all groups. T-suppressor cell microparticle numbers were decreased in normal pregnancy (P =.04). In preeclampsia T-suppressor, T-helper cell, and granulocyte microparticle numbers were increased (P =.008,.008, and.03, respectively). Elastase concentrations were increased in preeclampsia (P =.02) and correlated with granulocyte microparticle numbers (P =.006). Elastase concentrations correlated with systolic and diastolic blood pressure (P =.001 and.003, respectively), and granulocyte microparticle numbers correlated with systolic blood pressure (P =.05).
Numbers of T-cell and granulocyte microparticles are increased in preeclampsia. Whether these altered microparticle numbers cause vascular dysfunction in preeclampsia or are a consequence of the disease remains to be established.
本研究旨在调查正常妊娠和子痫前期循环微颗粒的细胞来源及数量。
采用流式细胞术分析来自10名单纯收缩期高血压患者、10名正常孕妇以及10名年龄和孕周匹配的非孕妇的血浆样本。
妊娠和子痫前期循环微颗粒的总数未发生改变。所有组中，微颗粒的最大部分来源于血小板。正常妊娠时抑制性T细胞微颗粒数量减少（P = 0.04）。子痫前期中，抑制性T细胞、辅助性T细胞和粒细胞微颗粒数量增加（分别为P = 0.008、0.008和0.03）。子痫前期中弹性蛋白酶浓度升高（P = 0.02），且与粒细胞微颗粒数量相关（P = 0.006）。弹性蛋白酶浓度与收缩压和舒张压相关（分别为P = 0.001和0.003），粒细胞微颗粒数量与收缩压相关（P = 0.05）。
子痫前期中T细胞和粒细胞微颗粒数量增加。这些改变的微颗粒数量是导致子痫前期血管功能障碍的原因还是该疾病的结果仍有待确定。
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