Liu Dong-Cheng, Gao Mu-Qiang, Guan Rong-Xia, Li Run-Zhi, Cao Shuang-He, Guo Xiao-Li, Zhang Ai-Min
Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100094, China.
Yi Chuan Xue Bao. 2002;29(8):706-11.
To detect quantitative trait loci (QTLs) controlling plant height, the plant height of 240 F2:3 lines derived from the cross of a dwarf wheat line ND3338 with a tall line F390, was assessed in field trials at two locations with three replications in 2000 and 2001. Microsatellite markers were used to construct a framework linkage map containing 215 loci with 21 linkage groups, and covering the whole genome about 3600cM. With the method of interval mapping, seven putative QTLs affecting plant height were detected on chromosomes 1B, 4B (two regions), 6A (two regions), 6D and 7A, respectively. Phenotypic variations explained by each QTL ranged from 5.2% to 50.1%, and in each environment the total putative QTLs explained about 64.8%-75% of the total phenotypic variation respectively. A major QTL located on chromosome arm 4BS near the locus Xgwm113, around the Rht-Blb locus, explained a large part of the phenotypic variation (27.8%-36.2% depending on the years or the locations). Except the QTL on chromosome 7A, all the other QTLs from ND3338 decreased the plant height, variously from 0.94 cm to 9.33 cm. Most of the identified QTLs were consistent in all the environments, and should be useful in future marker-assisted-selection programs for breeding dwarf and semi-dwarf wheat cultivars.
为检测控制株高的数量性状基因座（QTL），2000年和2001年在两个地点对240个源自矮秆小麦品系ND3338与高秆品系F390杂交的F2:3株系进行了田间试验，每个地点设置三次重复，测定其株高。利用微卫星标记构建了一个包含215个位点、21个连锁群、覆盖全基因组约3600厘摩（cM）的框架连锁图谱。采用区间作图法，在1B、4B（两个区域）、6A（两个区域）、6D和7A染色体上分别检测到7个影响株高的假定QTL。每个QTL解释的表型变异范围为5.2%至50.1%，在每个环境中，假定的QTL总共分别解释了约64.8% - 75%的总表型变异。位于4BS染色体臂上靠近Xgwm113位点、Rht - Blb位点附近的一个主效QTL解释了大部分表型变异（根据年份或地点不同，解释率为27.8% - 36.2%）。除了7A染色体上的QTL外，来自ND3338的所有其他QTL均降低了株高，降低幅度从0.94厘米到9.33厘米不等。大多数已鉴定的QTL在所有环境中都一致，对于未来矮秆和半矮秆小麦品种的标记辅助选择育种计划应该是有用的。
