通过荧光偏振对细胞表面进行荧光共振能量转移测量。
Fluorescence resonance energy transfers measurements on cell surfaces via fluorescence polarization.
作者信息
Cohen-Kashi Meir, Moshkov Sergey, Zurgil Naomi, Deutsch Mordechai
机构信息
The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome Physics Department, Bar-Ilan University, Ramat-Gan 52900, Israel.
出版信息
Biophys J. 2002 Sep;83(3):1395-402. doi: 10.1016/S0006-3495(02)73910-6.
A method has been developed for the determination of the efficiency of fluorescence resonance energy transfer efficiency between moieties located on cell surfaces by performing individual cell fluorescence polarization (FP) measurements. The absolute value of energy transfer efficiency (E) is calculated on an individual cell basis. The examination of this methodology was carried out using model experiments on human T lymphocyte cells. The cells were labeled with fluorescein-conjugated Concanavalin A (ConA) as donor, or rhodamine-conjugated ConA as acceptor. The experiments and results clearly indicate that determination of E via FP measurements is possible, efficient, and more convenient than other methods.
已开发出一种方法，通过进行单个细胞荧光偏振（FP）测量来测定位于细胞表面的部分之间的荧光共振能量转移效率。能量转移效率（E）的绝对值是在单个细胞基础上计算得出的。使用人T淋巴细胞的模型实验对该方法进行了检验。细胞用荧光素偶联的刀豆球蛋白A（ConA）作为供体进行标记，或用罗丹明偶联的ConA作为受体进行标记。实验和结果清楚地表明，通过FP测量来测定E是可行的、高效的，并且比其他方法更方便。
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