Dérue Cedric, Gibouin David, Verdus Marie-Claire, Lefebvre Fabrice, Demarty Maurice, Ripoll Camille, Thellier Michel
Laboratoire des Processus Intégratifs Cellulaires, UMR CNRS 6037, Faculté des Sciences de l'Université de Rouen, F-76821 Mont-Saint-Aignan Cedex, France.
Microsc Res Tech. 2002 Jul 15;58(2):104-10. doi: 10.1002/jemt.10125.
In the search for a new methodological approach applicable to the determination of the still poorly known primary role of boron in plant physiology, we have undertaken to appraise the potential of the SIMS method for the analytical imaging of the boron isotopes, (10)B and (11)B, at physiological concentrations in plants. With our own, CAMECA IMS4F SIMS ion analyser, and using O(2)(+) as primary ions for the detection of B(+) (plus (12)C(+) and (40)Ca(+)) secondary ions, we have been able to map quantitatively the two boron isotopes in control and boron-enriched plants, to evaluate boron concentrations at the level of individual cells and to determine boron isotopic ratios. This provides the opportunity to carry out the simultaneous labeling and imaging of boron, using enrichment with the stable isotopes, (10)B and (11)B. The method has also the potential for the simultaneous, quantitative detection of the boron isotopes and of the borate-binding sites in plant cells.
在寻找一种适用于确定硼在植物生理学中仍知之甚少的主要作用的新方法时，我们着手评估二次离子质谱法（SIMS）对植物生理浓度下硼同位素（¹⁰B和¹¹B）进行分析成像的潜力。使用我们自己的CAMECA IMS4F SIMS离子分析仪，并以O₂⁺作为初级离子来检测B⁺（以及¹²C⁺和⁴⁰Ca⁺）二次离子，我们已经能够对对照植物和富硼植物中的两种硼同位素进行定量成像，评估单个细胞水平的硼浓度，并确定硼同位素比率。这为利用稳定同位素¹⁰B和¹¹B富集进行硼的同时标记和成像提供了机会。该方法还具有同时定量检测植物细胞中硼同位素和硼酸盐结合位点的潜力。
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