基于单光子检测的新型DNA测序仪家族。
A family of novel DNA sequencing instruments based on single-photon detection.
作者信息
Alaverdian Ludmila, Alaverdian Sinok, Bilenko Olga, Bogdanov Iouri, Filippova Elena, Gavrilov Dmitry, Gorbovitski Boris, Gouzman Michael, Gudkov Georgy, Domratchev Sergey, Kosobokova Olga, Lifshitz Nadia, Luryi Serge, Ruskovoloshin Victor, Stepoukhovitch Andrew, Tcherevishnick Marina, Tyshko Georgy, Gorfinkel Vera
机构信息
Department of Electrical and Computer Engineering, State University of New York-Stony Brook, Stony Brook, NY 11794-2350, USA.
出版信息
Electrophoresis. 2002 Aug;23(16):2804-17. doi: 10.1002/1522-2683(200208)23:16<2804::AID-ELPS2804>3.0.CO;2-9.
We have developed a family of high-performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the "color-blind" single-photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.
我们基于一种新型多色荧光检测技术开发了一系列高性能毛细管DNA测序仪。该技术基于两项技术创新：标记DNA片段荧光的多激光激发以及调制荧光的“色盲”单光子检测。我们的仪器采用了现代数字和宽带技术，这些技术对于实现卓越的仪器性能至关重要。我们讨论了多个版本的自动单通道DNA测序仪的设计和测试结果，以及我们扩展到多通道仪器的方法。
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