小鼠结膜相关淋巴组织的组织学研究

Histological study of conjunctiva-associated lymphoid tissue in mouse.

作者信息

Sakimoto Tohru, Shoji Jun, Inada Noriko, Saito Keiko, Iwasaki Yutaka, Sawa Mitsuru

机构信息

Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan.

出版信息

Jpn J Ophthalmol. 2002 Jul-Aug;46(4):364-69. doi: 10.1016/s0021-5155(02)00503-8.

DOI:10.1016/s0021-5155(02)00503-8

PMID:12225813

Abstract

PURPOSE

To investigate conjunctiva-associated lymphoid tissue (CALT) in the mouse conjunctiva by histological methods.

METHODS

The presumed follicular tissue in the conjunctiva of normal mice, age ranging from 4 to 6 weeks, was histologically investigated by the hematoxylin-eosin staining method. Next, we treated the mice with topical instillation of a combined solution of ovalbumin and cholera toxin B to investigate the morphological changes of conjunctival follicles to antigen challenge. The treated mice underwent sequential clinical examinations, and the conjunctival follicular tissue was examined by an immunohistochemical method using anti-CD4 antibody, anti-CD8 antibody, and anti-S-100 antibody.

RESULTS

Follicular tissue was present on the mouse nictitating membrane. Both size and number of follicular tissue areas increased with topical ovalbumin treatment. Immunohistochemical study revealed CD4, CD8, and S-100 positive cells in the follicular tissue. The epithelial layer, corresponding to follicular tissue, demonstrated intra-epithelial pocket and the presence of CD4-positive cells in the intra-epithelial pocket.

CONCLUSION

Follicular tissue at the nictitating membrane is CALT in the mouse.

摘要

目的

通过组织学方法研究小鼠结膜中的结膜相关淋巴组织（CALT）。

方法

采用苏木精-伊红染色法对4至6周龄正常小鼠结膜中的假定滤泡组织进行组织学研究。接下来，我们通过局部滴注卵清蛋白和霍乱毒素B的混合溶液处理小鼠，以研究结膜滤泡对抗原刺激的形态学变化。对处理后的小鼠进行连续的临床检查，并使用抗CD4抗体、抗CD8抗体和抗S-100抗体通过免疫组织化学方法检查结膜滤泡组织。