氯丙嗪、己酮可可碱和地塞米松对脂多糖诱导的牛外周血单个核细胞炎性细胞因子mRNA表达的影响

Effects of chlorpromazine, pentoxifylline and dexamethasone on mRNA expression of lipopolysaccharide-induced inflammatory cytokines in bovine peripheral blood mononuclear cells.

作者信息

Kiku Yoshio, Matsuzawa Hironori, Ohtsuka Hiromichi, Terasaki Nobuhiro, Fukuda Shigeo, Kon-Nai Satoru, Koiwa Masateru, Yokomizo Yuichi, Sato Hiroshi, Rosol Thomas J, Okada Hiroyuki, Yoshino Tomo-o

机构信息

Department of Veterinary Pathology, large animal Clincal Center, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.

出版信息

J Vet Med Sci. 2002 Aug;64(8):723-6. doi: 10.1292/jvms.64.723.

DOI:10.1292/jvms.64.723

PMID:12237520

Abstract

The effects of chlorpromazine (CPZ), pentoxifylline (PTX) and dexamethasone (DEX) on mRNA expression of lipopolysaccharide (LPS)-induced proinflammatory cytokines were examined in bovine peripheral blood mononuclear cells (PBMCs) in vitro. The expression of inflammatory cytokine mRNAs was analyzed by RT-PCR and Southern blot hybridization in bovine PBMCs. CPZ and DEX decreased the expression of cytokine mRNA (such as interleukin-1 beta and tumor necrosis factor-alpha) after stimulation with LPS in a dose-dependent manner. However, pretreatment with PTX had no inhibitory effect on the mRNA expression of proinflammatory cytokines. These results indicated that pretreatment with CPZ and DEX might be effective to reduce the production of LPS-induced inflammatory cytokines in bovine PBMCs in vitro.

摘要

在体外对牛外周血单个核细胞（PBMCs）研究了氯丙嗪（CPZ）、己酮可可碱（PTX）和地塞米松（DEX）对脂多糖（LPS）诱导的促炎细胞因子mRNA表达的影响。通过逆转录聚合酶链反应（RT-PCR）和Southern印迹杂交分析牛PBMCs中炎性细胞因子mRNA的表达。CPZ和DEX在LPS刺激后以剂量依赖性方式降低细胞因子mRNA（如白细胞介素-1β和肿瘤坏死因子-α）的表达。然而，PTX预处理对促炎细胞因子的mRNA表达没有抑制作用。这些结果表明，CPZ和DEX预处理可能有效地减少体外培养的牛PBMCs中LPS诱导的炎性细胞因子的产生。