Kurita Atsushi, Kikuchi Shiro, Kakita Akira
Department of Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara-shi, Kanagawa 228, Japan.
Hepatogastroenterology. 2002 Sep-Oct;49(47):1235-8.
BACKGROUND/AIMS: The mechanisms involved in the unique stromal change that occurs upon cancer invasion are poorly understood in scirrhous carcinoma of the stomach.
Three different human gastric cancer cell lines (KATO-III, MKN-28, MKN-45) and human fibroblast cells (TIG-101) were co-cultured three-dimensionally in collagen gels. The gels, in vitro models of gastric cancer, were immunostained by monoclonal antibodies to human placental prolyl 4-hydroxylase (PH, a key enzyme of collagen synthesis) and then examined by light and electron microscopy.
Under co-culture of cancer cells and fibroblasts, cytoplasmic staining for PH was observed in both the cancer cells (KATO-III, MKN-28, MKN-45) and fibroblasts. No significant difference in the expression patterns of the alpha- and beta-subunits of PH was observed not only among the KATO-III, MKN-28 and MKN-45 cell lines but also between cancer cells and fibroblasts.
These findings indicate that both cancer cells and fibroblasts can synthesize collagen in gastric cancer models. Moreover, this property may not be a unique characteristic of scirrhous gastric cancer cells, but common to gastric cancer cells and fibroblasts in vitro.
背景/目的：在胃硬癌中，对于癌症侵袭时发生的独特基质变化所涉及的机制了解甚少。
将三种不同的人胃癌细胞系（KATO-III、MKN-28、MKN-45）与人成纤维细胞系（TIG-101）在胶原凝胶中进行三维共培养。这些凝胶作为胃癌的体外模型，用抗人胎盘脯氨酰4-羟化酶（PH，胶原合成的关键酶）的单克隆抗体进行免疫染色，然后通过光学显微镜和电子显微镜检查。
在癌细胞与成纤维细胞共培养时，在癌细胞（KATO-III、MKN-28、MKN-45）和成纤维细胞中均观察到PH的细胞质染色。不仅在KATO-III、MKN-28和MKN-45细胞系之间，而且在癌细胞与成纤维细胞之间，PH的α亚基和β亚基的表达模式均未观察到显著差异。
这些发现表明，在胃癌模型中癌细胞和成纤维细胞都能合成胶原。此外，这种特性可能不是胃硬癌细胞的独特特征，而是体外胃癌细胞和成纤维细胞共有的特征。
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