成年大鼠脑中γ-氨基丁酸转氨酶和谷氨酸脱氢酶的亚细胞定位。脑中至少存在两个小的谷氨酸区室的证据。
Subcellular localization of gamma-aminobutyrate transaminase and glutamate dehydrogenase in adult rat brain. Evidence for at least two small glutamate compartments in brain.
作者信息
Reijnierse G L, Veldstra H, Van den Berg C J
出版信息
Biochem J. 1975 Dec;152(3):469-75. doi: 10.1042/bj1520469.
The subcellular localizations of gamma-aminobutyrate transaminase (EC 2.6.1.19) and glutamate dehydrogenase (EC 1.4.1.2) in brain tissue of adult rats were compared with each other and with those of NAD+-isocitrate dehydrogenase (EC 1.1.41) and monoamine oxidase (EC 1.4.3.4; kynuramine as substrate). Crude mitochondrial fractions from brain tissue were centrifuged in continuous sucrose density gradients. gamma-Aminobutyrate transaminase and glutamate dehydrogenase were always found at a higher density than NAD+-isocitrate dehydrogenase and monoamine oxidase. When centrifuged for 1 h at 53 000gav., there was a slight difference between the distribution profiles of glutamate dehydrogenase and gamma-aminobutyrate transaminase. This difference was larger when the centrifugation time was only 15 min. It is concluded that there are subpopulations of brain mitochondria with differing proportions of gamma-aminobutyrate transaminase and glutamate dehydrogenase. The results are discussed in relation to evidence obtained with labelled precursors in vivo that there are at least two small glutamate compartments in adult brain.
将成年大鼠脑组织中γ-氨基丁酸转氨酶（EC 2.6.1.19）和谷氨酸脱氢酶（EC 1.4.1.2）的亚细胞定位相互进行了比较，并与NAD⁺-异柠檬酸脱氢酶（EC 1.1.41）和单胺氧化酶（EC 1.4.3.4；以犬尿胺为底物）的亚细胞定位进行了比较。对脑组织的粗线粒体部分在连续蔗糖密度梯度中进行离心。γ-氨基丁酸转氨酶和谷氨酸脱氢酶的密度总是高于NAD⁺-异柠檬酸脱氢酶和单胺氧化酶。当在53000gav下离心1小时时，谷氨酸脱氢酶和γ-氨基丁酸转氨酶的分布图谱之间存在细微差异。当离心时间仅为15分钟时，这种差异更大。得出的结论是，脑线粒体存在亚群，其中γ-氨基丁酸转氨酶和谷氨酸脱氢酶的比例不同。结合体内用标记前体获得的证据对结果进行了讨论，该证据表明成年大脑中至少存在两个小的谷氨酸区室。
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