土耳其献血者及一组患者中TTV病毒感染情况与基因型分布

TT virus infection and genotype distribution in blood donors and a group of patients from Turkey.

作者信息

Erensoy S, Sayiner A A, Türkoğlu S, Canatan D, Akarca U S, Sertöz R, Ozacar T, Batur Y, Badur S, Bilgiç A

机构信息

Dept. of Microbiology and Clinical Microbiology, Medical Faculty, Ege University, Bornova, 35100 Izmir, Turkey.

出版信息

Infection. 2002 Oct;30(5):299-302. doi: 10.1007/s15010-002-2185-z.

DOI:10.1007/s15010-002-2185-z

PMID:12382090

Abstract

BACKGROUND

TT virus (TTV) DNA has been found in a large proportion of patients with different forms of non-A-G hepatitis, however the clinical importance is unclear. We aimed to determine the genotypes of TTV isolates found in blood donors and different patient groups from the western part of Turkey.

MATERIALS AND METHODS

TT DNA was investigated in serum samples of 91 volunteer blood donors (BD), 105 thalassemia (TH) patients, ten patients with fulminant hepatitis (FH) and 16 hemodialysis (HD) patients by heminested PCR using primers NG059, NG061 and NG063 from the ORF1 region. 39 isolates were genotyped by analyzing the partial sequence of ORF1.

RESULTS

TTV DNA was found in 75% of HD, 80% of FH, 61% of TH patients and in 51.6% of BD. Among the sequenced isolates, 14 (35.9%) belonged to genotype 1 (G1) and 25 (64.1%) belonged to genotype 2 (G2). Among the G2 sequences, 22 were grouped as G2c.

CONCLUSION

TTV infection was common in the population studied, even with moderately sensitive primers. G2 was the major genotype of the studied population without any significant differences in distribution between various patient groups and BD.

摘要

背景

在大部分不同形式的非甲非戊型肝炎患者中都发现了TTV病毒（TTV）DNA，但其临床重要性尚不清楚。我们旨在确定在土耳其西部的献血者和不同患者群体中发现的TTV分离株的基因型。

材料与方法

采用巢式PCR技术，使用来自开放阅读框1（ORF1）区域的引物NG059、NG061和NG063，对91名志愿献血者（BD）、105名地中海贫血（TH）患者、10名暴发性肝炎（FH）患者和16名血液透析（HD）患者的血清样本进行TTV DNA检测。通过分析ORF1的部分序列对39个分离株进行基因分型。