[米非司酮增强卵巢细胞系对顺铂的敏感性及其机制]

[Enhanced sensitivity of ovarian cell line to cisplatin induced by mifepristone and its mechanism].

作者信息

Qin Tang-Ni, Wang Li-Li

机构信息

Department of Obstetrics and Gynecology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.

出版信息

Di Yi Jun Yi Da Xue Xue Bao. 2002 Apr;22(4):344-6.

PMID:12390742

Abstract

OBJECTIVE

To observe the effect of mifepristone in enhancing chemosensitivity of drug-resistant ovarian cancer cell line to cisplatin and investigate its mechanism.

METHODS

Human ovarian cancer cell lines COC1 (DDP-sensitive) and COC1/DDP (DDP-resistant) were incubated with or without mifepristone. The proliferation rate of the cells was determined by methyl thiazolyl tetrazolium (MTT) assay and positive expression of apoptosis-associated proteins Bcl-2 and Bax were observed by means of flow cytometry.

RESULTS

It was observed that mifepristone at the dose of 1.25 micromol/L reversed the resistance of COC1/DDP cells to cisplatin, and Bcl-2 protein expression was deceased from (23.8067+/-0.4382)% to (19.3967+/-0.6866)% (P=0.000) while Bax protein expression increased from (12.75+/-0.2524)% to (25.5967+/-0.8834)% (P=0.000).

CONCLUSION

Mifepristone may act to enhance the sensitivity of COC1/DDP cells to cisplatin, possibly through regulating Bcl-2 and Bax protein expressions.

摘要

目的

观察米非司酮增强耐药卵巢癌细胞系对顺铂化疗敏感性的作用并探讨其机制。

方法

将人卵巢癌细胞系COC1（顺铂敏感）和COC1/DDP（顺铂耐药）分别在有或无米非司酮的情况下进行培养。采用甲基噻唑基四氮唑（MTT）法测定细胞增殖率，通过流式细胞术观察凋亡相关蛋白Bcl-2和Bax的阳性表达情况。

结果

观察到1.25微摩尔/升剂量的米非司酮可逆转COC1/DDP细胞对顺铂的耐药性，Bcl-2蛋白表达从（23.8067±0.4382）%降至（19.3967±0.6866）%（P = 0.000），而Bax蛋白表达从（12.75±0.2524）%增至（25.5967±0.8834）%（P = 0.000）。