Sugiyama Atsushi, Maruta Fukuto, Tsuzuki Shigetoshi, Murakami Maki, Kawasaki Seiji
First Department of Surgery, Shinshu University, School of Medicine, Matsumoto, Japan.
Hepatogastroenterology. 2002 Nov-Dec;49(48):1734-6.
BACKGROUND/AIMS: Thymidine phosphorylase was reported to be identical to the angiogenic factor, platelet-derived endothelial cell growth factor. In this study we investigated the distribution of thymidine phosphorylase activity in human gastric carcinoma or normal gastric tissue using the ELISA system.
A longitudinal slice in the center of the gastric carcinoma of resected specimens from 6 patients with gastric carcinoma was used, and thymidine phosphorylase activity was mapped in each case.
In all cases, the thymidine phosphorylase activities were significantly higher in tumors than adjacent normal gastric tissues. The amount and distribution of thymidine phosphorylase activity were different between intestinal-type and diffuse-type carcinoma. The thymidine phosphorylase activities in the invasive front of tumor were significantly lower than those in the other part in intestinal-type carcinoma.
The ELISA system used in this study proved useful for the determination of thymidine phosphorylase activities in tissue sections.
背景/目的：据报道，胸苷磷酸化酶与血管生成因子血小板衍生内皮细胞生长因子相同。在本研究中，我们使用酶联免疫吸附测定（ELISA）系统研究了胸苷磷酸化酶活性在人胃癌组织和正常胃组织中的分布情况。
使用6例胃癌患者切除标本胃癌中心的纵向切片，对每例标本进行胸苷磷酸化酶活性定位。
在所有病例中，肿瘤组织中的胸苷磷酸化酶活性均显著高于相邻的正常胃组织。肠型癌和弥漫型癌中胸苷磷酸化酶活性的数量和分布有所不同。在肠型癌中，肿瘤浸润前沿的胸苷磷酸化酶活性显著低于其他部位。
本研究中使用的ELISA系统被证明可用于测定组织切片中的胸苷磷酸化酶活性。
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