可溶性耐药相关钙结合蛋白（索辛）基因在白血病患者中的表达及临床意义

Expression and clinical implications of the soluble drug resistance-related calcium-binding protein (sorcin) gene in leukemia patients.

作者信息

Li Guangyao, Tan Yaohong, Yang Chunzheng, Zhao Chunhua, Zhao Hongguo, Wang Jianxiang, Xue Yanping, Han Mingzhe, Qian Linsheng, Zhao Chunting

机构信息

Institute of Hematology, CAMS & PUMC, State Key Laboratory of Experimental Hematology, Tianjin 300020, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2002 Jun;23(6):293-6.

PMID:12411058

Abstract

OBJECTIVE

To study the relationship between the expression of soluble drug resistance-related calcium-binding protein (sorcin) gene and the clinical multidrug resistance in acute leukemia (AL).

METHODS

A semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was used to investigate the transcription levels of the human sorcin gene in 95 AL patients and 27 controls.

RESULTS

Sorcin gene expression was significantly higher in AL patients than in normal contrls (P < 0.001), and higher in relapsed/refractory acute myeloid leukemia (AML) patients than in those newly diagnosed or in complete remission. Sorcin gene overexpression was significantly lower in non-resistant patients than in resistant ones (P < 0.001). CR rates of these two groups were 20.0% and 80.0%, respectively. Sorcin gene expression was higher in AML-M(5) patients than M(2), M(3), M(4) patients.

CONCLUSION

Sorcin gene overexpression is significantly associated with clinical multidrug resistance and prognosis, it is one of the indicators for predicting prognosis of AL patients.

摘要

目的

研究可溶性耐药相关钙结合蛋白（Sorcin）基因表达与急性白血病（AL）临床多药耐药的关系。

方法

采用半定量逆转录聚合酶链反应（RT-PCR）检测95例AL患者和27例对照者中人类Sorcin基因的转录水平。

结果

Sorcin基因在AL患者中的表达显著高于正常对照（P<0.001），在复发/难治性急性髓系白血病（AML）患者中的表达高于新诊断或完全缓解的患者。Sorcin基因过表达在非耐药患者中显著低于耐药患者（P<0.001）。这两组的完全缓解率分别为20.0%和80.0%。Sorcin基因在AML-M(5)患者中的表达高于M(2)、M(3)、M(4)患者。