利用K-252a通过多倍体化获得的四倍体细胞建立三倍体V79细胞系。
Establishment of a triploid V79 cell line from tetraploid cells obtained through polyploidization using K-252a.
作者信息
Fujikawa-Yamamoto Kohzaburo, Yamagishi Hiroko, Miyagoshi Minoru
机构信息
Division of Basic Science, Research Institute of Medical Science, Kanazawa Medical University, Ishikawa, Japan.
出版信息
Cell Prolif. 2002 Dec;35(6):369-79. doi: 10.1046/j.1365-2184.2002.00250.x.
Abstract
Triploid V79 cells were established from tetraploid cells. Diploid V79 cells were polyploidized by K-252a, an inhibitor of protein kinases, and then released from the drug for 10 days. At that time, the cell population was a mixture of diploid and tetraploid cells. Triploid cells were obtained through the cloning of tetraploid cells. They had 33 chromosomes (1.5 times the diploid number) and showed a karyotype of three homologueous chromosomes. The duration of the G1, S and G2/M phases was almost the same as for diploid cells. The cell volume of triploid V79 cells was about two times that of the diploid cells. An explanation for the diploid-tetraploid-triploid transition is proposed.
摘要
三倍体V79细胞由四倍体细胞构建而成。二倍体V79细胞用蛋白激酶抑制剂K-252a进行多倍体化处理，然后停药10天。此时，细胞群体是二倍体细胞和四倍体细胞的混合物。通过克隆四倍体细胞获得三倍体细胞。它们有33条染色体（是二倍体染色体数的1.5倍），显示出三条同源染色体的核型。G1、S和G2/M期的持续时间与二倍体细胞几乎相同。三倍体V79细胞的细胞体积约为二倍体细胞的两倍。本文提出了二倍体-四倍体-三倍体转变的一种解释。
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