Cousin Sydney L, Whittington William L H, Roberts Marilyn C
Department of Pathobiology, University of Washington, Box 357238, School of Public Health and Community Medicine, Seattle, WA 98195, USA.
J Antimicrob Chemother. 2003 Jan;51(1):131-3. doi: 10.1093/jac/dkg040.
The presence of macrolide-lincosamide-streptogramin B resistance genes erm(B), erm(C) and erm(F), the macrolide resistance mef(A) gene, and the DNA sequence of a 13 bp repeat in the promoter region of the mtrR gene, were determined in 62 Neisseria gonorrhoeae isolates collected between 1992 and 1999 in Seattle, Washington, USA. Eleven isolates with erythromycin and azithromycin MICs of < or =0.06 mg/L, had no acquired genes or deletions in the 13 bp repeat region. Among 44 isolates with erythromycin MICs 1.0-16.0 mg/L, and azithromycin MICs 0.06-4.0 mg/L, 16 carried the 1 bp deletion in the mtrR promoter region alone, nine carried one or more of the four acquired macrolide resistance genes alone, and 14 carried both acquired macrolide resistance genes plus the 1 bp deletion in the mtrR promoter region. Three isolates with erythromycin MICs > or = 8 mg/L, and azithromycin MICs of 4.0 mg/L, carried only erm genes. Five isolates with MICs of 1-2 mg/L did not carry the 1 bp deletion, or any of the acquired resistance genes examined. Our data suggest that the 1 bp deletion in the mtrR promoter region is not found in all erythromycin-resistant (MIC > or = 1.0 mg/L) N. gonorrhoeae.
在美国华盛顿州西雅图市1992年至1999年间收集的62株淋病奈瑟菌中，检测了大环内酯-林可酰胺-链阳菌素B耐药基因erm(B)、erm(C)和erm(F)、大环内酯耐药mef(A)基因以及mtrR基因启动子区域13 bp重复序列的DNA序列。11株红霉素和阿奇霉素MIC≤0.06 mg/L的菌株，在13 bp重复区域未发现获得性基因或缺失。在44株红霉素MIC为1.0 - 16.0 mg/L、阿奇霉素MIC为0.06 - 4.0 mg/L的菌株中，16株仅在mtrR启动子区域存在1 bp缺失，9株仅携带四种获得性大环内酯耐药基因中的一种或多种，14株同时携带获得性大环内酯耐药基因以及mtrR启动子区域的1 bp缺失。3株红霉素MIC≥8 mg/L、阿奇霉素MIC为4.0 mg/L的菌株仅携带erm基因。5株MIC为1 - 2 mg/L的菌株未携带1 bp缺失或所检测的任何获得性耐药基因。我们的数据表明，并非所有红霉素耐药（MIC≥1.0 mg/L）的淋病奈瑟菌都存在mtrR启动子区域的1 bp缺失。
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