宫内注射大鼠胎儿肝细胞对大鼠肝移植排斥反应的影响

Influence of intrauterine injection of rat fetal hepatocytes on rejection of rat liver transplantation.

作者信息

Yang Yan-Ling, Dou Ke-Feng, Li Kai-Zong

机构信息

Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 710032 Xi'an, Shaanxi Province, China.

出版信息

World J Gastroenterol. 2003 Jan;9(1):137-40. doi: 10.3748/wjg.v9.i1.137.

DOI:10.3748/wjg.v9.i1.137

PMID:12508369

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4728228/

Abstract

AIM

To investigate the influence of immune tolerance induced by intrauterine exposure to fetal hepatocytes on liver transplantation in the adult rat.

METHODS

LOU/CN rat fetal hepatocytes were injected into the fetuses of pregnant CHN rats (14-16 days of gestation). At 7-9 weeks of age, the surviving male rats received orthotopic liver transplantation (OLT) from male LOU/CN donors and the survival period was observed and monitered by mixed lymphocyte reaction assay and cytotoxicity test.

RESULTS

(1) A total of 31 pregnant CHN rats with 172 fetuses received fetal hepatocytes from LOU/CN rats via intrauterine injection. Among them, thirteen pregnant rats showed normal parturition, with 74 neonatal rats growing up normally. (2) The mean survival period after OLT in rats with fetal exposure to fetal hepatocytes was 32.1+/-3.7 days, which was significantly different from the control (11.8+/-2.3 days, P<0.01) in rats without fetal induction of immune tolerance. (3) Mixed lymphocyte proliferation assays yielded remarkable discrepancies between the groups of rats with- or without fetal exposure to fetal hepatocytes, with values of 8,411+/-1,361 and 22,473+/-1,856 (CPM+/-SD, P<0.01) respectively. (4) Cytotoxicity assays showed values of 21.2+/-6.5 % and 64.5+/-7.2 % (P<0.01) in adult rats with or without fetal induction of immune tolerance.

CONCLUSION

Intrauterine injection of fetal hepatocytes into rat fetuses can prolong the survival period of liver transplant adult male rats recipients, inducting immune tolerance in OLT.

摘要

目的

研究宫内暴露于胎肝细胞诱导的免疫耐受对成年大鼠肝移植的影响。

方法

将LOU/CN大鼠胎肝细胞注入妊娠CHN大鼠（妊娠14 - 16天）的胎儿体内。7 - 9周龄时，存活的雄性大鼠接受来自雄性LOU/CN供体的原位肝移植（OLT），通过混合淋巴细胞反应测定和细胞毒性试验观察并监测生存期。

结果

（1）共有31只妊娠CHN大鼠，172只胎儿经宫内注射接受了LOU/CN大鼠的胎肝细胞。其中，13只妊娠大鼠正常分娩，74只新生大鼠正常生长。（2）胎肝细胞暴露的大鼠OLT术后平均生存期为32.1±3.7天，与未诱导免疫耐受的对照大鼠（11.8±2.3天，P<0.01）有显著差异。（3）混合淋巴细胞增殖试验显示，胎肝细胞暴露组与未暴露组大鼠之间存在显著差异，分别为8411±1361和22473±1856（CPM±SD，P<0.01）。（4）细胞毒性试验显示，诱导免疫耐受的成年大鼠和未诱导免疫耐受的成年大鼠的值分别为21.2±6.5%和64.5±7.2%（P<0.01）。