Sung M H, Kim H, Bae J W, Rhee S K, Jeon C O, Kim K, Kim J J, Hong S P, Lee S G, Yoon J H, Park Y H, Baek D H
Int J Syst Evol Microbiol. 2002 Nov;52(Pt 6):2251-2255. doi: 10.1099/00207713-52-6-2251.
A thermophilic, spore-forming rod isolated from hay compost in Korea was subjected to a taxonomic study. The micro-organism, designated strain SK-1(T), was identified as being aerobic, Gram-positive, motile and rod-shaped. Growth of the isolate was observed at 45-70 degrees C (optimum 60 degrees C) and pH 6.0-9.0 (optimum pH 7.5). The G+C content of the genomic DNA was 43.9 mol%. Chemotaxonomic characteristics of the isolate included the presence of mesodiaminopimelic acid in the cell wall and iso-C15:0 and iso-C17:0 as the major cellular fatty acids. The predominant isoprenoid quinone was MK-7. The chemotaxonomic characteristics of strain SK-1(T) were the same as those of the genus Geobacillus. Phylogenetic analysis based on 16S rDNA sequences showed that strain SK-1(T) is most closely related to Geobacillus thermoglucosidasius. However, the phenotypic properties of strain SK-1(T) were clearly different from those of G. thermoglucosidasius. The level of DNA-DNA relatedness between strain SK-1(T) and the type strain of G. thermoglucosidasius was 27%. On the basis of the phenotypic traits and molecular systematic data, strain SK-1(T) represents a novel species within the genus Geobacillus, for which the name Geobacillus toebii sp. nov. is proposed. The type strain is strain SK-1(T) (= KCTC 0306BP(T) - DSM 14590(T)).
从韩国干草堆肥中分离出的一株嗜热、产芽孢杆菌进行了分类学研究。该微生物被命名为SK-1(T)菌株，鉴定为需氧、革兰氏阳性、具运动性且呈杆状。在45-70℃（最适温度60℃）和pH 6.0-9.0（最适pH 7.5）条件下观察到该分离株的生长。基因组DNA的G+C含量为43.9 mol%。该分离株的化学分类学特征包括细胞壁中存在内消旋二氨基庚二酸以及以异-C15:0和异-C17:0作为主要细胞脂肪酸。主要类异戊二烯醌为MK-7。SK-1(T)菌株的化学分类学特征与嗜热栖热菌属相同。基于16S rDNA序列的系统发育分析表明，SK-1(T)菌株与嗜热葡糖苷酶栖热菌最为密切相关。然而，SK-1(T)菌株的表型特性与嗜热葡糖苷酶栖热菌明显不同。SK-1(T)菌株与嗜热葡糖苷酶栖热菌模式菌株之间的DNA-DNA相关性水平为27%。基于表型特征和分子系统学数据，SK-1(T)菌株代表嗜热栖热菌属内的一个新物种，为此提出新名称嗜热栖热菌托氏新种。模式菌株为SK-1(T)菌株（=KCTC 0306BP(T)-DSM 14590(T)）。
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