Dhar Kajari, Dhar Alok, Rosazza John P N
Division of Medicinal and Natural Products Chemistry, Center for Biocatalysis and Bioprocessing, College of Pharmacy, The University of Iowa, Iowa City, Iowa 52242, USA.
Appl Environ Microbiol. 2003 Jan;69(1):707-10. doi: 10.1128/AEM.69.1.707-710.2003.
An inducible, cytosolic glutathione S-transferase (GST) was purified from Streptomyces griseus. GST isoenzymes with pI values of 6.8 and 7.9 used standard GST substrates including 1-chloro-2,4-dinitrobenzene. GST had subunit and native M(r)s of 24 and 48, respectively, and the N-terminal sequence SMILXYWDIIRGLPAH.
从灰色链霉菌中纯化出一种可诱导的胞质谷胱甘肽S-转移酶(GST)。pI值为6.8和7.9的GST同工酶使用包括1-氯-2,4-二硝基苯在内的标准GST底物。GST的亚基分子量和天然分子量分别为24和48,其N端序列为SMILXYWDIIRGLPAH。