小球藻的冷冻保存。1. 冷却速率、保护性添加剂与升温速率之间的相互作用。

The cryopreservation of Chlorella. 1. Interactions of rate of cooling, protective additive and warming rate.

作者信息

Morris G J

出版信息

Arch Microbiol. 1976 Feb;107(1):57-62. doi: 10.1007/BF00427867.

PMID:1252089

Abstract

The cryoprotective additives glycerol and dimethylsulphoxide were found to be toxic to Chlorella cells at concentrations greater then 2.5% w/v. Polyvinylpyrrolidone, was not damaging up to a concentration of 15%w/v. Chlorella 211/7a had a recovery rate greater than 95% at all rates of cooling studied. With Chlorella 211/8h the survival was lower than 0.1% at all rates examined. The addition of dimethylsulphoxide (5% w/v) to Chlorella 211/8h increased the recovery, particularly at the faster rates of cooling; with polyvinylpyrrolidone (10% w/v) there was an optimum range of cooling rate. Cells of Chlorella 211/7a from the exponential phase of growth were found to be damaged both by a temperature reduction from 25 degrees C to 0 degrees C (thermal shock) and by freezing and thawing. In contrast cells from the stationary phase of growth were resistant to these stresses.

摘要

研究发现，当冷冻保护添加剂甘油和二甲基亚砜的浓度高于2.5%（w/v）时，它们对小球藻细胞有毒性。聚乙烯吡咯烷酮在浓度高达15%（w/v）时不会造成损害。在所有研究的冷却速率下，小球藻211/7a的复苏率均大于95%。对于小球藻211/8h，在所有检测的速率下，其存活率均低于0.1%。向小球藻211/8h中添加二甲基亚砜（5%，w/v）可提高复苏率，尤其是在较快的冷却速率下；添加聚乙烯吡咯烷酮（10%，w/v）时，存在一个最佳冷却速率范围。处于生长指数期的小球藻211/7a细胞，在温度从25℃降至0℃（热休克）以及冻融过程中均受到损伤。相比之下，处于生长稳定期的细胞对这些应激具有抗性。