用于DNA测序的点击化学构建荧光寡核苷酸。
Click chemistry to construct fluorescent oligonucleotides for DNA sequencing.
作者信息
Seo Tae Seok, Li Zengmin, Ruparel Hameer, Ju Jingyue
机构信息
Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
出版信息
J Org Chem. 2003 Jan 24;68(2):609-12. doi: 10.1021/jo026615r.
"Click chemistry" 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.
在水性条件下，进行了炔基6-羧基荧光素（FAM）与叠氮基标记的单链（ss）DNA之间的“点击化学”1,3-偶极环加成反应，以定量产率生成FAM标记的ssDNA。FAM标记的ssDNA成功用作引物，在具有激光诱导荧光检测的毛细管电泳DNA测序仪中产生具有单碱基分辨率的DNA测序产物。
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