[葫芦素BE聚乳酸纳米粒靶向颈淋巴结的急性毒性及局部刺激性试验]

[Acute toxicity and local stimulate test of cucurbitacinBE polylactic acid nano-particles of targeting cervical lymph nodes].

作者信息

Yang K, Wen Y, Li L, Wang C, Wang X

机构信息

Department of Stomatology, First Affiliated Hospital, Chongqing Medical University.

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2001 Dec;19(6):380-2.

PMID:12539689

Abstract

OBJECTIVE

The aim of this experiment was to study acute toxicity and local stimulation of cucurbitacinBE poly-lactic acid nano-particles (CuBE-PLA-NP) for targeting cervical lymph node.

METHODS

Various doses of CuBE-PLA-NP lyophilization injection and cucurbitacinBE (CuBE) were hypodermically or veno-injected into the mice, and then the drug toxicity and side effects were observed; 50% lethal dose (LD50) was counted. CuBE-PLA-NP lyophilization injection and CuBE were also injected into the quadriceps femoralis muscle of rabbits to observe the local stimulate responses of drugs.

RESULTS

The fifty percent lethal doses of subcutaneous and venous CuBE-PLA-NP in mice were 16.74 mg/kg +/- 1.83 mg/kg and 5.60 mg/kg +/- 0.83 mg/kg respectively, that were higher than those of CuBE which were 8.63 mg/kg +/- 1.00 mg/kg and 2.50 mg/kg +/- 0.30 mg/kg respectively. Local stimulate response in rabbit quadriceps femoralis muscle after CuBE-PLA-NP and CuBE injected are 0 grade and 0.1 grade respectively.

CONCLUSION

The acute toxicity of CuBE-PLA-NP is lower than that of CuBE. Both CuBE-PLA-NP and CuBE are adequate for local injection, but the local stimulation of CuBE-PLA-NP is smaller than that of CuBE.

摘要

目的

本实验旨在研究葫芦素BE聚乳酸纳米粒（CuBE-PLA-NP）靶向颈淋巴结的急性毒性和局部刺激性。

方法

将不同剂量的CuBE-PLA-NP冻干注射液和葫芦素BE（CuBE）皮下或静脉注射到小鼠体内，观察药物毒性和副作用；计算半数致死量（LD50）。将CuBE-PLA-NP冻干注射液和CuBE也注射到兔股四头肌中，观察药物的局部刺激反应。

结果

小鼠皮下和静脉注射CuBE-PLA-NP的半数致死量分别为16.74 mg/kg±1.83 mg/kg和5.60 mg/kg±0.83 mg/kg，高于CuBE的半数致死量，CuBE皮下和静脉注射的半数致死量分别为8.63 mg/kg±1.00 mg/kg和2.50 mg/kg±0.30 mg/kg。注射CuBE-PLA-NP和CuBE后兔股四头肌的局部刺激反应分别为0级和0.1级。