反相高效液相色谱法测定四环素类抗生素

[Determination of tetracyclines antibiotics by reversed-phase high performance liquid chromatography].

作者信息

Wang Lei, Zhang Xiao-song, Xu Zhi-xiu, Shao Xue-guang

机构信息

Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.

出版信息

Se Pu. 2002 Jan;20(1):49-51.

PMID:12541619

Abstract

A method for the determination of 7 tetracycline antibiotics (TCs) by reversed-phase high performance liquid chromatography is described. TCs were successfully separated on a Diamonsil C18 column (250 mm x 4.6 mm i.d., 5 microns) using methanol-acetonitrile-0.01 mol/L oxalic acid (pH 2.0) (11:22:67, volume ratio) as mobile phase at a flow rate of 0.8 mL/min and detected at 267 nm within 22 min. Effects of the pH value of the mobile phase, concentration of the mobile phase buffer, elution composition and detection wavelength on the response and retention were studied. Tetracycline (TC) and oxytetracycline(OTC) in medicinal tablets were quantitated by standard added method. It has been proven that the method is fast, accurate and suitable for routine analysis.

摘要

描述了一种用反相高效液相色谱法测定7种四环素类抗生素（TCs）的方法。使用Diamonsil C18柱（250 mm×4.6 mm内径，5微米），以甲醇 - 乙腈 - 0.01 mol/L草酸（pH 2.0）（体积比11:22:67）为流动相，流速为0.8 mL/min，在267 nm处检测，22分钟内成功分离了TCs。研究了流动相pH值、流动相缓冲液浓度、洗脱成分和检测波长对响应和保留的影响。采用标准加入法对药片中的四环素（TC）和土霉素（OTC）进行定量。结果表明该方法快速、准确，适用于常规分析。