使用冷冻断裂和负染色技术研究从B16黑色素瘤中分离出的黑素小体的超微结构。

Lea P J, Haberman H F, Pawlowski A, Menon I A

J Anat. 1976 Feb;121(Pt 1):1-5.

Freeze-fracture negative staining methods were used to investigate the structure of melanosomes isolated from B16 melanoma. The findings demonstrated the presence of a melanosomal double-layered membrane. Negative staining procedure showed that the melanosomal membrane is sometimes ruptured, displaced or peeled off during the purification process. Fractured melanosomes showed melanin spheres with a mean diameter of 24 nm. This compares with a mean diameter of 26 nm for the melanin spheres revealed by negative staining. The granular appearance of fractured mature melanosomes is due to the fracture following a random path between melanin spheres.

采用冷冻断裂负染色法研究从B16黑色素瘤中分离出的黑素小体的结构。研究结果表明存在黑素小体双层膜。负染色程序显示，在纯化过程中黑素小体膜有时会破裂、移位或剥离。断裂的黑素小体显示出平均直径为24nm的黑色素球。相比之下，负染色显示的黑色素球平均直径为26nm。断裂的成熟黑素小体的颗粒状外观是由于在黑色素球之间随机路径断裂所致。