戊二酸诱导幼鼠大脑中的氧化应激。
Glutaric acid induces oxidative stress in brain of young rats.
作者信息
de Oliveira Marques Fernanda, Hagen Martine Elisabeth Kienzle, Pederzolli Carolina Didonet, Sgaravatti Angela Malysz, Durigon Karina, Testa Carla Giordani, Wannmacher Clóvis Milton Duval, de Souza Wyse Angela Terezinha, Wajner Moacir, Dutra-Filho Carlos Severo
机构信息
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, Porto Alegre RS 90035-003, Brazil.
出版信息
Brain Res. 2003 Feb 21;964(1):153-8. doi: 10.1016/s0006-8993(02)04118-5.
This study investigated the effects of glutaric acid, which predominantly accumulates in glutaric acidemia type I, on some in vitro parameters of oxidative stress in brain of young rats. We evaluated chemiluminescence, total radical-antioxidant potential (TRAP) and the activities of the antioxidant enzymes catalase, glutathione peroxidase and superoxide dismutase in brain tissue homogenates in the presence of glutaric acid at concentrations ranging from 0.05 to 2.0 mM. The acid significantly increased chemiluminescence (up to 65%) and reduced total radical-antioxidant potential (up to 28%) and glutathione peroxidase activity (up to 46%), without affecting catalase and superoxide dismutase activities. The results provide evidence that glutaric acid induces oxidative stress in vitro in rat brain. If these findings also occur in humans, it is possible that they may contribute to the neuropathology of patients affected by glutaric acidemia type I.
本研究考察了主要在I型戊二酸血症中蓄积的戊二酸对幼鼠大脑氧化应激一些体外参数的影响。我们评估了在浓度范围为0.05至2.0 mM的戊二酸存在下，脑组织匀浆中的化学发光、总自由基抗氧化能力（TRAP）以及抗氧化酶过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的活性。该酸显著增加化学发光（高达65%）并降低总自由基抗氧化能力（高达28%）和谷胱甘肽过氧化物酶活性（高达46%），而不影响过氧化氢酶和超氧化物歧化酶的活性。结果提供了证据表明戊二酸在体外可诱导大鼠大脑氧化应激。如果这些发现也出现在人类中，则有可能它们会促成受I型戊二酸血症影响患者的神经病理学改变。
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