Li Y, Feng G, Yi Y, Zhong X, Zheng H
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Yan Ke Xue Bao. 1999 Dec;15(4):218-20, 269.
To study the healing of the corneal endothelial cells in rabbit corneal alkali wounds.
26 New Zealand white rabbits were used. A alkali wound was created with a round filter paper, 8 mm in diameter, which was soaked in 0.5 N NaOH for 5 seconds and was placed centrally on the cornea for 60 seconds. The rabbits were killed in 20 minutes; 1, 2, 6, 12 h; 1, 2, 3, 5, 7, 10, 14, 30 days after injury. The corneal endothelium was examined in the light microscope using a lizarin red S-trypan blue staining method.
Twenty minutes after the wound, the corneal endothelial cells were destroyed. 72 hours following the wound, the endothelial cells surrounding the burns became larger, irregular and started to migrate onto the denuded descemet's membrane. The endothelial cells covered the burn with the spindle-shaped cells 7 days after wound.
The endothelial damage is healed by the division and migration of remaining endothelial cells at the margin of the wound, which has some characters of fibroblast.
研究兔角膜碱烧伤后角膜内皮细胞的愈合情况。
选用26只新西兰白兔。用直径8mm的圆形滤纸浸于0.5N氢氧化钠中5秒制成碱烧伤模型，将滤纸置于角膜中央60秒。分别于伤后20分钟、1、2、6、12小时及1、2、3、5、7、10、14、30天处死动物。采用茜素红S-台盼蓝染色法在光学显微镜下观察角膜内皮细胞。
伤后20分钟角膜内皮细胞被破坏。伤后72小时，烧伤周边的内皮细胞变大、形态不规则，并开始向裸露的后弹力层迁移。伤后7天内皮细胞以梭形细胞覆盖烧伤区。
角膜内皮损伤是通过伤口边缘剩余内皮细胞的分裂和迁移而愈合的，这些内皮细胞具有成纤维细胞的一些特征。
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