分离几丁质分解菌的新方法及其在筛选木糖氧化产碱菌高几丁质酶产生突变体中的应用。

The novel method for isolating chitinolytic bacteria and its application in screening for hyperchitinase producing mutant of Alcaligenes xylosoxydans.

作者信息

Vaidya R J, Macmil S L A, Vyas P R, Chhatpar H S

机构信息

Department of Microbiology and Biotechnology Centre, Faculty of Science, MS University of Baroda, Vadodara, Gujarat, India.

出版信息

Lett Appl Microbiol. 2003;36(3):129-34. doi: 10.1046/j.1472-765x.2003.01274.x.

DOI:10.1046/j.1472-765x.2003.01274.x

PMID:12581369

Abstract

AIMS

To develop a novel, rapid and effective screening method for chitinase producing bacteria.

METHODS AND RESULTS

A simple and rapid technique for screening of potential chitinolytic bacteria has been developed using the chitin binding dye calcofluor white M2R in chitin agar. Microorganisms possessing high chitinolytic potential gave a clear zone under ultraviolet light after 24-48 h of incubation. This method was successfully applied for isolating the hyperchitinase mutant of Alcaligenes xylosoxydans. The mutant Alc. xylosoxydans EMS 33 was found to produce 3.4 times more chitinase than the wild type.

CONCLUSIONS

In this study, the screening method for chitinase producing bacteria has been developed and it was applied to screen chitinase-overproducing mutant of Alc. xylosoxydans.

SIGNIFICANCE AND IMPACT OF THE STUDY

The novel screening method for chitinase producer is more sensitive, rapid, user-friendly and reliable, which can also be used for screening of recombinants having chitinase gene.

摘要

目的

开发一种用于筛选产几丁质酶细菌的新型、快速且有效的筛选方法。

方法与结果

利用几丁质琼脂中的几丁质结合染料荧光增白剂M2R，开发了一种简单快速的筛选潜在几丁质分解细菌的技术。具有高几丁质分解潜力的微生物在培养24 - 48小时后，在紫外线下会出现清晰的区域。该方法成功应用于分离木糖氧化产碱杆菌的高几丁质酶突变体。发现突变体木糖氧化产碱杆菌EMS 33产生的几丁质酶比野生型多3.4倍。