[二丁酰环磷酸腺苷对精子活力的影响]
[Influence of dibutyryl cyclic adenosine monophosphate on motility of sperm].
作者信息
Li Yang, Liu Ji-Hong
机构信息
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
出版信息
Zhonghua Nan Ke Xue. 2002 Dec;8(6):398-400.
OBJECTIVES
To investigate the influence of cAMP/PKA signal transduction on human sperm motility, and to study the effect of dibutyryl cyclic adenosine monophosphate dibutyryl cyclic adenosine monophosphate (dbcAMP) on human sperm motility in vitro.
METHODS
Sperm aseptically obtained by masturbation and prepared by swim-up technique from 10 healthy fertile men were incubated with different concentrations of dbcAMP. Measurement of mobility were carried out at 20, 30, and 60 min in all specimens.
RESULTS
The sperm treated with dbcAMP showed a significant increase in sperm progressive motility and the percentage of motile cells. The effect seemed enhanced with the increasing of dbcAMP concentration. VSL and VCL were not affected by dbcAMP.
CONCLUSIONS
dbcAMP can activate the mobility of human sperm in vitro.
目的
研究环磷酸腺苷/蛋白激酶A（cAMP/PKA）信号转导对人精子活力的影响，并探讨二丁酰环磷腺苷（dbcAMP）对体外人精子活力的作用。
方法
从10名健康有生育能力的男性中通过手淫无菌获取精子，并采用上游法制备，将其与不同浓度的dbcAMP孵育。在所有样本的20、30和60分钟时测量精子活力。
结果
用dbcAMP处理的精子在进行性运动和活动细胞百分比方面显著增加。随着dbcAMP浓度的增加，这种作用似乎增强。直线运动速度（VSL）和曲线运动速度（VCL）不受dbcAMP影响。
结论
dbcAMP可在体外激活人精子的活力。
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