[恶性成釉细胞瘤的定量病理学研究]
[A quantitatively pathological study on malignant ameloblastomas].
作者信息
Liu Jinzhong, Wang Shuozhi, Chen Xinming
机构信息
College of Stomatology, Wuhan University.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2002 Jun;20(3):187-9.
OBJECTIVE
The purpose of this study was to investigate the role of nuclear morphometric parameters, DNA content and Ag-NOR count in the differentiating malignant and benign ameloblastomas.
METHODS
Totally 17 cases of malignant ameloblastomas were examined by using HE, AgNOR and DNA stain methods. Morphometric parameters of cell nuclei, DNA content and AgNOR count were quantitatively studied by using an image analysis system.
RESULTS
Seven parameters (area, perimeter, equal diameter, minor diameter, mean diameter, round index, axis ratio) out of ten shape factors were significantly different between malignant and benign ameloblastoma (P < 0.01). AgNOR count and DNA index in malignant ameloblastoma were significantly higher than those in benign ameloblastoma (P < 0.01). Logistic regression equation was established, according to nuclear morphormetric parameters and DNA index.
CONCLUSION
Quantitative analysis of DAN content, nuclear morphmetric parameters and AgNOR count may be helpful in differentiating malignant and benign ameloblastomas.
目的
本研究旨在探讨细胞核形态计量参数、DNA含量和银染核仁组织区计数在鉴别恶性和良性成釉细胞瘤中的作用。
方法
采用苏木精-伊红（HE）染色、银染核仁组织区（AgNOR）染色及DNA染色方法，对17例恶性成釉细胞瘤进行检测。利用图像分析系统对细胞核形态计量参数、DNA含量及AgNOR计数进行定量研究。
结果
在十个形态因子中，有七个参数（面积、周长、等直径、短径、平均直径、圆形指数、轴比）在恶性和良性成釉细胞瘤之间存在显著差异（P < 0.01）。恶性成釉细胞瘤的AgNOR计数和DNA指数显著高于良性成釉细胞瘤（P < 0.01）。根据细胞核形态计量参数和DNA指数建立了逻辑回归方程。
结论
DNA含量、细胞核形态计量参数和AgNOR计数的定量分析可能有助于鉴别恶性和良性成釉细胞瘤。
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