Song Hai-yan, Yang Qing-en, Yu Chun-ying
Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030.
Fa Yi Xue Za Zhi. 2002 Aug;18(3):152-4.
PGM1 genotyping by PCR-SSCP analysis.
Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes.
2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318. PCR-SSCP analysis was suitable for determination of PGM1 genotypes from old blood and semen stains.
PGM1 system typed by PCR-SSCP is useful for forensic identification.
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