Nagaraja P, Vasantha R A, Yathirajan H S
Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, India.
J Pharm Biomed Anal. 2003 Mar 10;31(3):563-9. doi: 10.1016/s0731-7085(02)00465-x.
A simple, sensitive and selective spectrophotometric method for the determination of indomethacin (INM) either in pure form or in capsules is described. The method is based on the coupling reaction of hydrolyzed INM with diazotized p-phenylenediamine dihydrochloride (PPDD) in sulphuric acid medium to give a red coloured product having the absorption maximum at 510 nm. The product is stable for 20 h. Beer's law is obeyed in the concentration range of 0.2-10 microg/ml. Results of the proposed method compare favourably with those of the official methods and offer the merits of sensitivity and stability. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method.
本文描述了一种简单、灵敏且具选择性的分光光度法，用于测定纯吲哚美辛（INM）或其胶囊剂。该方法基于在硫酸介质中，水解后的INM与重氮化的对苯二胺二盐酸盐（PPDD）发生偶合反应，生成一种在510 nm处有最大吸收的红色产物。该产物在20小时内稳定。在0.2 - 10微克/毫升的浓度范围内符合比尔定律。所提方法的结果与官方方法的结果相比具有优势，且具有灵敏度高和稳定性好的优点。药物制剂中用作添加剂的常见辅料在所提方法中不产生干扰。
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