Courtright J B
J Bacteriol. 1975 Oct;124(1):497-502. doi: 10.1128/jb.124.1.497-502.1975.
A mutant of Neurospora crassa able to grow on liquid minimal glycerol medium without evidence of conidiation and with high cell yields has been isolated and shown to be allelic to ff-1. The glycerol-specific induction of glycerokinase and glycerol-3-phosphate dehydrogenase was similar in both wild-type and mutant cells, although higher specific activities as well as higher glycerokinase cross-reacting material levels were found in fully induced mutant cells. After growth in minimal glycerol medium there is a significant reduction in wild-type cells of the activities of both pyruvate dehydrogenase and dihydrolipoyl transacetylase. This evidence indicates a relationship between the conditional acetate requirement by wild-type cells grown on glycerol medium and the levels of the pyruvate dehydrogenase complex.
已分离出一种粗糙脉孢菌突变体，它能够在液体基本甘油培养基上生长，不产生分生孢子且细胞产量高，并且已证明它与ff-1等位。甘油激酶和甘油-3-磷酸脱氢酶的甘油特异性诱导在野生型和突变体细胞中相似，尽管在完全诱导的突变体细胞中发现了更高的比活性以及更高的甘油激酶交叉反应物质水平。在基本甘油培养基中生长后，野生型细胞中丙酮酸脱氢酶和二氢硫辛酰转乙酰酶的活性均显著降低。这一证据表明，在甘油培养基上生长的野生型细胞对乙酸盐的条件需求与丙酮酸脱氢酶复合体的水平之间存在关联。
