五种血清学检测方法在诊断幼驹马红球菌肺炎中的性能。
Performance of five serological assays for diagnosis of Rhodococcus equi pneumonia in foals.
作者信息
Giguère Steeve, Hernandez Jorge, Gaskin Jack, Prescott John F, Takai Shinji, Miller Corey
机构信息
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610, USA.
出版信息
Clin Diagn Lab Immunol. 2003 Mar;10(2):241-5. doi: 10.1128/cdli.10.2.241-245.2003.
The performance of four enzyme-linked immunosorbent assays (ELISAs) (ELISA-6939, ELISA-33701, ELISA-VapA, and ELISA-California) and an agar gel immunodiffusion test for diagnosis of Rhodococcus equi pneumonia in foals was evaluated. Antibody concentrations of foals with culture-confirmed R. equi pneumonia (n = 41) were compared to those of age-matched pasturemates that remained clinically healthy during the entire breeding season (n = 24). For each serological assay evaluated, selection of a low cutoff resulted in high sensitivity but low specificity. Increasing the cutoff value resulted in better specificity but to the detriment of sensitivity. The best diagnostic performance was achieved with ELISA-California at a cutoff of 40%, resulting in a sensitivity of 59% and a specificity of 88%. We conclude that current serological assays do not differentiate between diseased and clinically healthy foals.
评估了四种酶联免疫吸附测定（ELISA）（ELISA - 6939、ELISA - 33701、ELISA - VapA和ELISA - California）以及琼脂凝胶免疫扩散试验在诊断幼驹马红球菌肺炎中的性能。将培养确诊为马红球菌肺炎的幼驹（n = 41）的抗体浓度与在整个繁殖季节临床健康的年龄匹配的同牧场幼驹（n = 24）的抗体浓度进行比较。对于所评估的每种血清学检测，选择低临界值会导致高灵敏度但低特异性。提高临界值会导致更好的特异性，但会损害灵敏度。ELISA - California在临界值为40%时具有最佳诊断性能，灵敏度为59%，特异性为88%。我们得出结论，目前的血清学检测无法区分患病和临床健康的幼驹。
Zotero 插件