弗氏植滴虫中的细胞外金属蛋白酶活性

Extracellular metalloproteinase activity in Phytomonas françai.

作者信息

Almeida Flávia V S, Branquinha Marta H, Giovanni-De-Simone Salvatore, Vermelho Alane B

机构信息

Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS Bloco I, 21941-590, Rio de Janeiro, Brazil.

出版信息

Parasitol Res. 2003 Mar;89(4):320-2. doi: 10.1007/s00436-002-0792-3. Epub 2002 Dec 17.

DOI:10.1007/s00436-002-0792-3

PMID:12632171

Abstract

Extracellular proteolytic activities were detected in Phytomonas françai culture supernatant. A 67-kDa enzyme was purified by ammonium sulfate precipitation and gel filtration in a HPLC system. This proteinase was optimally active at 28 degrees C and pH 5.0; and the use of proteolytic inhibitors indicated that it belongs to the metalloproteinase class. This is the first report on the purification of an extracellular metalloproteinase from a Phytomonas species.

摘要

在法氏植鞭毛虫培养上清液中检测到细胞外蛋白水解活性。通过硫酸铵沉淀和高效液相色谱系统中的凝胶过滤纯化出一种67 kDa的酶。这种蛋白酶在28℃和pH 5.0时活性最佳；蛋白水解抑制剂的使用表明它属于金属蛋白酶类。这是关于从植鞭毛虫属物种中纯化细胞外金属蛋白酶的首次报道。