台湾南部三种与食物中毒有关的新腹足纲（榧螺科）物种中的麻痹性毒素。

Paralytic toxins in three new gastropod (Olividae) species implicated in food poisoning in southern Taiwan.

作者信息

Hwang Pai-An, Tsai Yung-Hsiang, Lu Ya-Hui, Hwang Deng-Fwu

机构信息

Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 202, Taiwan, ROC.

出版信息

Toxicon. 2003 Mar;41(4):529-33. doi: 10.1016/s0041-0101(02)00364-1.

DOI:10.1016/s0041-0101(02)00364-1

PMID:12657324

Abstract

The toxins in the new gastropods Oliva miniacea, O. mustelina and O. nirasei implicated in a food paralytic poisoning incident in South Taiwan in February 2002 were studied. It was found that the three species of gastropods contained moderate amounts of toxin in edible portion only, and the highest toxicity score was 18 MU/g for O. miniacea, 10 MU/g for O. mustelina, and 27 MU/g for O. nirasei. The toxin was partially purified from the toxic specimens of each species by ultrafiltration using a YM-1 membrane, followed by chromatography on Bio-Gel P-2 column. Analyses by HPLC, GC-MS and LC-MS showed that the toxin from O. miniacea, O. nirasei and O. mustelina contained TTX, and related compounds 4-epi TTX and anhydro-TTX. The paralytic shellfish poisons were not found.

摘要

对2002年2月在台湾南部一起因食用贝类导致麻痹性中毒事件中涉及的新腹足纲动物橄榄螺（Oliva miniacea）、鼬背橄榄螺（O. mustelina）和拟枣贝（O. nirasei）所含毒素进行了研究。发现这三种腹足纲动物仅在可食用部分含有适量毒素，其中橄榄螺的最高毒性评分为18 MU/g，鼬背橄榄螺为10 MU/g，拟枣贝为27 MU/g。通过使用YM - 1膜超滤，然后在Bio - Gel P - 2柱上进行色谱分离，从每个物种的有毒标本中对毒素进行了部分纯化。通过高效液相色谱（HPLC）、气相色谱 - 质谱联用（GC - MS）和液相色谱 - 质谱联用（LC - MS）分析表明，橄榄螺、拟枣贝和鼬背橄榄螺所含毒素含有河豚毒素（TTX）以及相关化合物4 - 表河豚毒素（4 - epi TTX）和脱水河豚毒素（anhydro - TTX）。未发现麻痹性贝类毒素。